BMRB Entry 15547
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PDB ID: 2jx5
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15547
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Catic, Andre; Sun, Zhen-Yu; Ratner, Daniel; Misaghi, Shahram; Spooner, Eric; Samuelson, John; Wagner, Gerhard; Ploegh, Hidde. "Sequence and structure evolved separately in a ribosomal ubiquitin variant" EMBO J. 26, 3474-3483 (2007).
PubMed: 17599068
Assembly members:
GlUb(S27a), polymer, 69 residues, 7307.402 Da.
Natural source: Common Name: Giardia intestinalis Taxonomy ID: 5741 Superkingdom: Eukaryota Kingdom: not available Genus/species: Giardia lamblia
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
GlUb(S27a): MLVIVRLQDQTLPFELPAGA
RASQLSNLLSSSGMAFSLHT
QGRVLSEAAELNDKMVIDAF
VPADGAGLE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 265 |
| 15N chemical shifts | 66 |
| 1H chemical shifts | 456 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GlUb(S27a) | 1 |
Entities:
Entity 1, GlUb(S27a) 69 residues - 7307.402 Da.
two C-terminal residues are from cloning artifact
| 1 | MET | LEU | VAL | ILE | VAL | ARG | LEU | GLN | ASP | GLN | ||||
| 2 | THR | LEU | PRO | PHE | GLU | LEU | PRO | ALA | GLY | ALA | ||||
| 3 | ARG | ALA | SER | GLN | LEU | SER | ASN | LEU | LEU | SER | ||||
| 4 | SER | SER | GLY | MET | ALA | PHE | SER | LEU | HIS | THR | ||||
| 5 | GLN | GLY | ARG | VAL | LEU | SER | GLU | ALA | ALA | GLU | ||||
| 6 | LEU | ASN | ASP | LYS | MET | VAL | ILE | ASP | ALA | PHE | ||||
| 7 | VAL | PRO | ALA | ASP | GLY | ALA | GLY | LEU | GLU |
Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone
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