BMRB Entry 15644
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PDB ID: 2k0a
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15644
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: van Roon, Anne-Marie; Loening, Nikolaus; Obayashi, Eiji; Yang, Ji-Chun; Newman, Andrew; Hernandez, Helena; Nagai, Kiyoshi; Neuhaus, David. "Solution structure of the U2 snRNP protein Rds3p reveals a knotted zinc-finger motif" Proc. Natl. Acad. Sci. U. S. A. 105, 9621-9626 (2008).
PubMed: 18621724
Assembly members:
Rds3p, polymer, 109 residues, 12353.605 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRK172
Entity Sequences (FASTA):
Rds3p: GGSSRHQFDLIMCLKQPGVQ
TGLLCEKCDGKCPICDSYVR
PKRKVRVCENCSFGKQAKNC
IICNLNVGVNDAFYCWECCR
LGKDKDGCPRILNLGSNRLD
RHFEKKKKV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 479 |
| 15N chemical shifts | 109 |
| 1H chemical shifts | 756 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Rds3 | 1 |
| 2 | ZINC ION_1 | 2 |
| 3 | ZINC ION_2 | 2 |
| 4 | ZINC ION_3 | 2 |
Entities:
Entity 1, Rds3 109 residues - 12353.605 Da.
residues -1 to 1 (GGS) result from cleavage of an N-terminal GST fusion protein
| 1 | GLY | GLY | SER | SER | ARG | HIS | GLN | PHE | ASP | LEU | ||||
| 2 | ILE | MET | CYS | LEU | LYS | GLN | PRO | GLY | VAL | GLN | ||||
| 3 | THR | GLY | LEU | LEU | CYS | GLU | LYS | CYS | ASP | GLY | ||||
| 4 | LYS | CYS | PRO | ILE | CYS | ASP | SER | TYR | VAL | ARG | ||||
| 5 | PRO | LYS | ARG | LYS | VAL | ARG | VAL | CYS | GLU | ASN | ||||
| 6 | CYS | SER | PHE | GLY | LYS | GLN | ALA | LYS | ASN | CYS | ||||
| 7 | ILE | ILE | CYS | ASN | LEU | ASN | VAL | GLY | VAL | ASN | ||||
| 8 | ASP | ALA | PHE | TYR | CYS | TRP | GLU | CYS | CYS | ARG | ||||
| 9 | LEU | GLY | LYS | ASP | LYS | ASP | GLY | CYS | PRO | ARG | ||||
| 10 | ILE | LEU | ASN | LEU | GLY | SER | ASN | ARG | LEU | ASP | ||||
| 11 | ARG | HIS | PHE | GLU | LYS | LYS | LYS | LYS | VAL |
Entity 2, ZINC ION_1 - Zn - 65.409 Da.
| 1 | ZN |
Related Database Links:
| PDB | 2K0A |
| DBJ | GAA27055 |
| EMBL | CAY87048 |
| GB | AAB68140 AAT93199 AHY78252 AJP42220 AJV91308 |
| REF | NP_015419 |
| SP | Q06835 |
| TPG | DAA11509 |
| AlphaFold | Q06835 |
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SPARKY: Backbone
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