BMRB Entry 15645

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PDB ID: 2k0d
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15645
MolProbity Validation Chart

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Title:
NMR STRUCTURE OF A MUTANT COLICIN E7 IMMUNITY PROTEIN IM7 WITH AN EXTENDED HELIX III
Deposition date:
2008-02-01
Original release date:
2009-10-14
Authors:
Figueiredo, Angelo; Whittaker, Sara; Spronk, Chris; Knowling, Stuart; Radford, Sheena; Moore, Geoffrey
Citation:

Citation: Knowling, Stuart; Figueiredo, Angelo Miguel; Whittaker, Sara B-M; Moore, Geoffrey; Radford, Sheena. "Amino acid insertion reveals a necessary three-helical intermediate in the folding pathway of the colicin E7 immunity protein Im7."  J. Mol. Biol. 392, 1074-1086 (2009).
PubMed: 19651139

Assembly members:

Assembly members:
Im7_mutant, polymer, 101 residues, 10009.231 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTrc99A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Im7_mutant: MEHHHHHHELKNSISDYTEA EFVQLLKEIEKENVAATDDV LDVLLEHFVKITEHPDGTAL IYEAAARAAANPGGDGGGPE GIVKEIKEWRAANGKPGFKQ G

Data sets:
Data typeCount
13C chemical shifts403
15N chemical shifts95
1H chemical shifts641

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Im7_mutant1

Entities:

Entity 1, Im7_mutant 101 residues - 10009.231 Da.

1   METGLUHISHISHISHISHISHISGLULEU
2   LYSASNSERILESERASPTYRTHRGLUALA
3   GLUPHEVALGLNLEULEULYSGLUILEGLU
4   LYSGLUASNVALALAALATHRASPASPVAL
5   LEUASPVALLEULEUGLUHISPHEVALLYS
6   ILETHRGLUHISPROASPGLYTHRALALEU
7   ILETYRGLUALAALAALAARGALAALAALA
8   ASNPROGLYGLYASPGLYGLYGLYPROGLU
9   GLYILEVALLYSGLUILELYSGLUTRPARG
10   ALAALAASNGLYLYSPROGLYPHELYSGLN
11   GLY

Related Database Links:

BMRB 15666
PDB 2K0D
GB EYB47026

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