BMRB Entry 15659

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PDB ID: 2k0y
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15659
MolProbity Validation Chart

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Title:
The actinorhodin apo acyl carrier protein from S. coelicolor
Deposition date:
2008-02-15
Original release date:
2008-09-11
Authors:
Crump, Matthew; Evans, Simon; Christopher, Williams
Citation:

Citation: Evans, Simon; Williams, Christopher; Arthur, Christopher; Burston, Steven; Simpson, Thomas; Crosby, John; Crump, Matthew. "An ACP Structural Switch: Conformational Differences between the Apo and Holo Forms of the Actinorhodin Polyketide Synthase Acyl Carrier Protein"  ChemBioChem 9, 2424-2432 (2008).
PubMed: 18770515

Assembly members:

Assembly members:
act_ACP, polymer, 86 residues, 9239.254 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 1902   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptomyces coelicolor

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11c

Entity Sequences (FASTA):

Entity Sequences (FASTA):
act_ACP: MATLLTTDDLRRALVESAGE TDGTDLSGDFLDLRFEDIGY DSLALMETAARLESRYGVSI PDDVAGRVDTPRELLDLING ALAEAA

Data sets:
Data typeCount
13C chemical shifts362
15N chemical shifts92
1H chemical shifts577

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 86 residues - 9239.254 Da.

This sequence contains a C17S mutation to prevent dimerisation of the ACP under NMR conditions.

1   METALATHRLEULEUTHRTHRASPASPLEU
2   ARGARGALALEUVALGLUSERALAGLYGLU
3   THRASPGLYTHRASPLEUSERGLYASPPHE
4   LEUASPLEUARGPHEGLUASPILEGLYTYR
5   ASPSERLEUALALEUMETGLUTHRALAALA
6   ARGLEUGLUSERARGTYRGLYVALSERILE
7   PROASPASPVALALAGLYARGVALASPTHR
8   PROARGGLULEULEUASPLEUILEASNGLY
9   ALALEUALAGLUALAALA

Related Database Links:

BMRB 15658 16196 16197 16199 16200 16201 16202 16203 25284 25287
PDB 1AF8 2AF8 2K0X 2K0Y 2KG6 2KG8 2KG9 2KGA 2KGC 2KGD 2KGE 2MVU 2MVV
EMBL CAA45045 CAC44202
GB AIJ13577 EFD66960 EOY50075 KKD13304
REF NP_629239 WP_003973889
SP Q02054
AlphaFold Q02054

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks