BMRB Entry 15666

Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15666

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Title:
Mutant Collision E7 protein IM7 with an extended helix III in M Urea
Deposition date:
2008-02-20
Original release date:
2009-11-10
Authors:
Figueiredo, Angelo; Whittaker, Sara; Knowling, Stuart; Radford, Sheena; Moore, Geoffrey
Citation:

Citation: Knowling, Stuart; Figueiredo, Angelo Miguel; Whittaker, Sara B-M; Moore, Geoffrey; Radford, Sheena. "Amino acid insertion reveals a necessary three-helical intermediate in the folding pathway of the colicin E7 immunity protein Im7."  J. Mol. Biol. 392, 1074-1086 (2009).
PubMed: 19651139

Assembly members:

Assembly members:
Im7_mutant, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: JM109

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Im7_mutant: MEHHHHHHELKNSISDYTEA EFVQLLKEIEKENVAATDDV LDVLLEHFVKITEHPDGTAL IYEAAARAAANPGGDGGGPE GIVKEIKEWRAANGKPGFKQ G

Data sets:
Data typeCount
13C chemical shifts175
15N chemical shifts88
1H chemical shifts88

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Im7 mutant1

Entities:

Entity 1, Im7 mutant 101 residues - Formula weight is not available

1   METGLUHISHISHISHISHISHISGLULEU
2   LYSASNSERILESERASPTYRTHRGLUALA
3   GLUPHEVALGLNLEULEULYSGLUILEGLU
4   LYSGLUASNVALALAALATHRASPASPVAL
5   LEUASPVALLEULEUGLUHISPHEVALLYS
6   ILETHRGLUHISPROASPGLYTHRALALEU
7   ILETYRGLUALAALAALAARGALAALAALA
8   ASNPROGLYGLYASPGLYGLYGLYPROGLU
9   GLYILEVALLYSGLUILELYSGLUTRPARG
10   ALAALAASNGLYLYSPROGLYPHELYSGLN
11   GLY

Related Database Links:

BMRB 15645
PDB 2K0D
GB EYB47026

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks