Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15669
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Citation: He, Ju; Kutateladze, Tatiana. "Sequence-specific 1H, 13C, and 15N resonance assignments of the GRP1 PH domain" Biomol. NMR Assignments 2, 97-99 (2008).
PubMed: 19636878
Assembly members:
GRP1, polymer, 125 residues, 15373.4 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRSET A
Data type | Count |
13C chemical shifts | 475 |
15N chemical shifts | 124 |
1H chemical shifts | 882 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | PH domain of GRP1 | 1 |
Entity 1, PH domain of GRP1 125 residues - 15373.4 Da.
1 | THR | PHE | PHE | ASN | PRO | ASP | ARG | GLU | GLY | TRP | ||||
2 | LEU | LEU | LYS | LEU | GLY | GLY | ARG | VAL | LYS | THR | ||||
3 | TRP | LYS | ARG | ARG | TRP | PHE | ILE | LEU | THR | ASP | ||||
4 | ASN | CYS | LEU | TYR | TYR | PHE | GLU | TYR | THR | THR | ||||
5 | ASP | LYS | GLU | PRO | ARG | GLY | ILE | ILE | PRO | LEU | ||||
6 | GLU | ASN | LEU | SER | ILE | ARG | GLU | VAL | GLU | ASP | ||||
7 | PRO | ARG | LYS | PRO | ASN | CYS | PHE | GLU | LEU | TYR | ||||
8 | ASN | PRO | SER | HIS | LYS | GLY | GLN | VAL | ILE | LYS | ||||
9 | ALA | CYS | LYS | THR | GLU | ALA | ASP | GLY | ARG | VAL | ||||
10 | VAL | GLU | GLY | ASN | HIS | VAL | VAL | TYR | ARG | ILE | ||||
11 | SER | ALA | PRO | SER | PRO | GLU | GLU | LYS | GLU | GLU | ||||
12 | TRP | MET | LYS | SER | ILE | LYS | ALA | SER | ILE | SER | ||||
13 | ARG | ASP | PRO | PHE | TYR |
sample_1: GRP1, [U-15N], 1 ± 0.1 mM; NaCl 300 ± 0.1 mM
sample_2: GRP1, [U-13C; U-15N], 1 ± 0.1 mM; NaCl 300 ± 0.1 mM
sample_conditions_1: ionic strength: 0.3 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_2 | isotropic | sample_conditions_1 |
3D C(CO)NH | sample_2 | isotropic | sample_conditions_1 |
3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
3D H(CCO)NH | sample_2 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_2 | isotropic | sample_conditions_1 |
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
PDB | |
DBJ | BAA33433 BAC29342 BAD90330 BAE25057 BAE34915 |
EMBL | CAA06434 CAA11686 |
GB | AAB41445 AAB60876 AAF23858 AAH07189 AAH08191 |
REF | NP_001072881 NP_001157020 NP_001238790 NP_004218 NP_035312 |
SP | O08967 O43739 P97696 |
TPG | DAA15150 |
AlphaFold | O08967 O43739 P97696 |
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