BMRB Entry 15673
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PDB ID: 2k19
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15673
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Martin-Visscher, Leah; Sprules, Tara; Gursky, Lucas; Vederas, John. "Nuclear magnetic resonance solution structure of PisI, a group B immunity protein that provides protection against the type IIa bacteriocin piscicolin 126, PisA." Biochemistry 47, 6427-6436 (2008).
PubMed: 18500825
Assembly members:
PisI, polymer, 98 residues, 11197.856 Da.
Natural source: Common Name: Carnibacterium piscicola Taxonomy ID: 2751 Superkingdom: Bacteria Kingdom: not available Genus/species: Carnobacterium maltaromaticum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMAL
Entity Sequences (FASTA):
PisI: MGKLKWFSGGKERSNQAENI
ITDLLDDLKTDLDNESLKKV
LENYLEELKQKSASVPLILS
RMNLDISKAIRNDGVTLSDY
QSKKLKELTSISNIRYGY
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 342 |
| 15N chemical shifts | 106 |
| 1H chemical shifts | 674 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PisI | 1 |
Entities:
Entity 1, PisI 98 residues - 11197.856 Da.
| 1 | MET | GLY | LYS | LEU | LYS | TRP | PHE | SER | GLY | GLY | ||||
| 2 | LYS | GLU | ARG | SER | ASN | GLN | ALA | GLU | ASN | ILE | ||||
| 3 | ILE | THR | ASP | LEU | LEU | ASP | ASP | LEU | LYS | THR | ||||
| 4 | ASP | LEU | ASP | ASN | GLU | SER | LEU | LYS | LYS | VAL | ||||
| 5 | LEU | GLU | ASN | TYR | LEU | GLU | GLU | LEU | LYS | GLN | ||||
| 6 | LYS | SER | ALA | SER | VAL | PRO | LEU | ILE | LEU | SER | ||||
| 7 | ARG | MET | ASN | LEU | ASP | ILE | SER | LYS | ALA | ILE | ||||
| 8 | ARG | ASN | ASP | GLY | VAL | THR | LEU | SER | ASP | TYR | ||||
| 9 | GLN | SER | LYS | LYS | LEU | LYS | GLU | LEU | THR | SER | ||||
| 10 | ILE | SER | ASN | ILE | ARG | TYR | GLY | TYR |
Related Database Links:
| PDB | 2K19 |
| EMBL | CCO10376 |
| GB | AAK69418 AAX21353 KRN60250 KRN73764 KRN85081 |
| REF | WP_010050956 WP_056999692 |
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