BMRB Entry 15676

Name: NMR Studies of a Pathogenic Mutant (D178N) of the Human Prion Protein
Published: 2009-11-30

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PDB ID: 2k1d
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15676
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Studies of a Pathogenic Mutant (D178N) of the Human Prion Protein

Deposition date:

2008-02-29

Original release date:

2009-11-30

Authors:

Mills, Jeffrey; Surewicz, Krystyna; Surewicz, Witold; Sonnichsen, Frank

Citation:

Citation: Mills, Jeffrey; Surewicz, Krystyna; Surewicz, Witold; Sonnichsen, Frank. "Null" .

Assembly members:

Assembly members:
V129/D178N_prion_protein, polymer, 146 residues, 16471.2 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRSETB

Entity Sequences (FASTA):

Entity Sequences (FASTA):
V129/D178N_prion_protein: GSDPGQGGGTHSQWNKPSKP KTNMKHMAGAAAAGAVVGGL GGYVLGSAMSRPIIHFGSDY EDRYYRENMHRYPNQVYYRP MDEYSNQNNFVHNCVNITIK QHTVTTTTKGENFTETDVKM MERVVEQMCITQYERESQAY YQRGSS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	301
15N chemical shifts	117
1H chemical shifts	635

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	monomer	1

Entities:

Entity 1, monomer 146 residues - 16471.2 Da.

Expressed protein contained residues 90-231 (plus GSDP cloning artifact), but only residues 125-231 are deposited

1

GLY

SER

ASP

PRO

GLY

GLN

GLY

THR

2

HIS

SER

GLN

TRP

ASN

LYS

PRO

SER

LYS

PRO

3

LYS

THR

ASN

MET

LYS

HIS

MET

ALA

GLY

ALA

4

ALA

GLY

ALA

VAL

GLY

LEU

5

GLY

TYR

VAL

LEU

GLY

SER

ALA

MET

SER

6

ARG

PRO

ILE

HIS

PHE

GLY

SER

ASP

TYR

7

GLU

ASP

ARG

TYR

ARG

GLU

ASN

MET

HIS

8

ARG

TYR

PRO

ASN

GLN

VAL

TYR

ARG

PRO

9

MET

ASP

GLU

TYR

SER

ASN

GLN

ASN

PHE

10

VAL

HIS

ASN

CYS

VAL

ASN

ILE

THR

ILE

LYS

11

GLN

HIS

THR

VAL

THR

LYS

GLY

12

GLU

ASN

PHE

THR

GLU

THR

ASP

VAL

LYS

MET

13

MET

GLU

ARG

VAL

GLU

GLN

MET

CYS

ILE

14

THR

GLN

TYR

GLU

ARG

GLU

SER

GLN

ALA

TYR

15

TYR

GLN

ARG

GLY

SER

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

BMRB	16743 16757 17714 17756 17780 18426 18550 19268 4379 4434 4641
PDB	1E1G 1E1J 1E1P 1E1S 1E1U 1E1W 1FKC 1FO7 1HJM 1HJN 1I4M 1QM0 1QM1 1QM2 1QM3 2K1D 2KUN 2LEJ 2LFT 2LSB 2LV1 2M8T 2W9E 3HAF 3HAK 3HEQ 3HER 3HES 3HJ5 3HJX 4DGI 4N9O
DBJ	BAF62360 BAG32277 BAG32279 BAG35206 BAG52189
EMBL	CAG46869
GB	AAA68632 AAA68633 AAC50085 AAC50088 AAC50089
REF	NP_001009093 NP_001103676 XP_003278028 XP_003278029 XP_003278030
SP	P40252 P61766 P61767 P61768
AlphaFold	P40252 P61766 P61767 P61768

BMRB Entry 15676

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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