BMRB Entry 15763

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PDB ID: 2k3j
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15763
MolProbity Validation Chart

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Title:
The solution structure of human Mia40
Deposition date:
2008-05-08
Original release date:
2009-02-09
Authors:
Ciofi Baffoni, Simone; Bertini, Ivano; Gallo, Angelo
Citation:

Citation: Banci, Lucia; Bertini, Ivano; Cefaro, Chiara; Ciofi-Baffoni, Simone; Gallo, Angelo; Martinelli, Manuele; Dionisia, Sideris; Nitsa, Katrakili; Tokatlidis, Kostas. "MIA40 is an oxidoreductase that catalyzes oxidative protein folding in mitochondria"  Nat. Struct. Mol. Biol. 16, 198-206 (2009).
PubMed: 19182799

Assembly members:

Assembly members:
Human_Mia40, polymer, 146 residues, 15996.3 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pDEST/HIS-MBP

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Human_Mia40: GSFTMSYCRQEGKDRIIFVT KEDHETPSSAELVADDPNDP YEEHGLILPNGNINWNCPCL GGMASGPCGEQFKSAFSCFH YSTEEIKGSDCVDQFRAMQE CMQKYPDLYPQEDEDEEEER EKKPAEQAEETAPIEATATK EEEGSS

Data sets:
Data typeCount
13C chemical shifts240
15N chemical shifts66
1H chemical shifts393

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Human Mia401

Entities:

Entity 1, Human Mia40 146 residues - 15996.3 Da.

Residues 1-4 (not present in wild type gene) are non native as a consequence of gene cloning.

1   GLYSERPHETHRMETSERTYRCYSARGGLN
2   GLUGLYLYSASPARGILEILEPHEVALTHR
3   LYSGLUASPHISGLUTHRPROSERSERALA
4   GLULEUVALALAASPASPPROASNASPPRO
5   TYRGLUGLUHISGLYLEUILELEUPROASN
6   GLYASNILEASNTRPASNCYSPROCYSLEU
7   GLYGLYMETALASERGLYPROCYSGLYGLU
8   GLNPHELYSSERALAPHESERCYSPHEHIS
9   TYRSERTHRGLUGLUILELYSGLYSERASP
10   CYSVALASPGLNPHEARGALAMETGLNGLU
11   CYSMETGLNLYSTYRPROASPLEUTYRPRO
12   GLNGLUASPGLUASPGLUGLUGLUGLUARG
13   GLULYSLYSPROALAGLUGLNALAGLUGLU
14   THRALAPROILEGLUALATHRALATHRLYS
15   GLUGLUGLUGLYSERSER

Related Database Links:

BMRB 17067
PDB 2K3J 2L0Y
DBJ BAB71132 BAF83453
GB AAH17082 AAH33775 AIC53061 EAW64180 EAW64181
REF NP_001091972 NP_001185638 NP_653237 XP_001157417 XP_002813171
SP Q8N4Q1
AlphaFold Q8N4Q1

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