BMRB Entry 15863

Title:
Solution structure of EAS D15 truncation mutant
Deposition date:
2008-07-07
Original release date:
2008-09-24
Authors:
Kwan, Ann
Citation:

Citation: Kwan, Ann; Macindoe, Ingrid; Paul, Vukasin; Morris, Vanessa; Kass, Itamar; Gupte, Rima; Mark, Alan; Templeton, Matthew; Mackay, Joel; Sunde, Margaret. "The Cys3-Cys4 loop of the hydrophobin EAS is not required for rodlet formation and surface activity"  J. Mol. Biol. 382, 708-720 (2008).
PubMed: 18674544

Assembly members:

Assembly members:
EAS_D15, polymer, 68 residues, 6818.720 Da.

Natural source:

Natural source:   Common Name: Neurospora crassa   Taxonomy ID: 5141   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Neurospora crassa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHUE

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts72
1H chemical shifts417

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1EAS_D151

Entities:

Entity 1, EAS_D15 68 residues - 6818.720 Da.

Residue 1 (Ser) represents a non-native residue from cloning.

1   SERALATHRTHRILEGLYPROASNTHRCYS
2   SERILEASPASPTYRLYSPROTYRCYSCYS
3   GLNSERMETSERGLYSERALASERLEUGLY
4   CYSVALVALGLYVALILEGLYSERGLNCYS
5   GLYALASERVALLYSCYSCYSLYSASPASP
6   VALTHRASNTHRGLYASNSERPHELEUILE
7   ILEASNALAALAASNCYSVALALA

Related Database Links:

SWS Q04571
BMRB 17765
PDB 2K6A 2LFN

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks