BMRB Entry 15912
Click here to enlarge.
PDB ID: 2k79
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15912
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Severin, Andrew; Joseph, Raji; Boyken, Scott; Fulton, Donald; Andreotti, Amy. "Proline isomerization preorganizes the Itk SH2 domian for binding to the Itk SH3 domain" J. Mol. Biol. 387, 726-743 (2009).
PubMed: 19361414
Assembly members:
Itk_SH3_domain, polymer, 64 residues, 7356.008 Da.
Itk_SH2_domain, polymer, 117 residues, 12534.314 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pgex2T
Entity Sequences (FASTA):
Itk_SH3_domain: GSPEETLVIALYDYQTNDPQ
ELALRCDEEYYLLDSSEIHW
WRVQDKNGHEGYAPSSYLVE
KSPN
Itk_SH2_domain: GSNNLETYEWYNKSISRDKA
EKLLLDTGKEGAFMVRDSRT
PGTYTVSVFTKAIISENPCI
KHYHIKETNDSPKRYYVAEK
YVFDSIPLLIQYHQYNGGGL
VTRLRYPVCGSPGIHRD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 1384 |
| 15N chemical shifts | 374 |
| 1H chemical shifts | 2178 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Itk_SH3_domain | 1 |
| 2 | Itk_SH2_domain | 2 |
Entities:
Entity 1, Itk_SH3_domain 64 residues - 7356.008 Da.
GS is the remnant from GST tag cleavage.
| 1 | GLY | SER | PRO | GLU | GLU | THR | LEU | VAL | ILE | ALA | ||||
| 2 | LEU | TYR | ASP | TYR | GLN | THR | ASN | ASP | PRO | GLN | ||||
| 3 | GLU | LEU | ALA | LEU | ARG | CYS | ASP | GLU | GLU | TYR | ||||
| 4 | TYR | LEU | LEU | ASP | SER | SER | GLU | ILE | HIS | TRP | ||||
| 5 | TRP | ARG | VAL | GLN | ASP | LYS | ASN | GLY | HIS | GLU | ||||
| 6 | GLY | TYR | ALA | PRO | SER | SER | TYR | LEU | VAL | GLU | ||||
| 7 | LYS | SER | PRO | ASN |
Entity 2, Itk_SH2_domain 117 residues - 12534.314 Da.
GS is the remnant from GST tag cleavage. GS in this protein fragment is not visible by NMR.
| 1 | GLY | SER | ASN | ASN | LEU | GLU | THR | TYR | GLU | TRP | ||||
| 2 | TYR | ASN | LYS | SER | ILE | SER | ARG | ASP | LYS | ALA | ||||
| 3 | GLU | LYS | LEU | LEU | LEU | ASP | THR | GLY | LYS | GLU | ||||
| 4 | GLY | ALA | PHE | MET | VAL | ARG | ASP | SER | ARG | THR | ||||
| 5 | PRO | GLY | THR | TYR | THR | VAL | SER | VAL | PHE | THR | ||||
| 6 | LYS | ALA | ILE | ILE | SER | GLU | ASN | PRO | CYS | ILE | ||||
| 7 | LYS | HIS | TYR | HIS | ILE | LYS | GLU | THR | ASN | ASP | ||||
| 8 | SER | PRO | LYS | ARG | TYR | TYR | VAL | ALA | GLU | LYS | ||||
| 9 | TYR | VAL | PHE | ASP | SER | ILE | PRO | LEU | LEU | ILE | ||||
| 10 | GLN | TYR | HIS | GLN | TYR | ASN | GLY | GLY | GLY | LEU | ||||
| 11 | VAL | THR | ARG | LEU | ARG | TYR | PRO | VAL | CYS | GLY | ||||
| 12 | SER | PRO | GLY | ILE | HIS | ARG | ASP |
Related Database Links:
| PDB | 2rna 1AWJ 2K79 2K7A 2RN8 2RNA 1luk 1lun 1LUI 1LUK 1LUM 1LUN 2ETZ 2EU0 3S9K |
| BMRB | 11018 16809 16809 5461 |
| DBJ | BAA03129 BAE32118 BAA03129 BAC29830 BAE32118 |
| GB | AAA39337 AAA40518 AAI28375 AAI28376 EDL33821 AAA39337 AAA40518 AAI28375 AAI28376 EDL33821 |
| REF | NP_001102295 NP_001268894 NP_001268895 NP_001268896 NP_001268897 NP_001102295 NP_001268894 NP_001268895 NP_001268897 NP_034713 |
| SP | Q03526 Q03526 |
| AlphaFold | Q03526 Q03526 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks