BMRB Entry 15946
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15946
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Title: Solution structure of a paralog-specific Mena binder by NMR PubMed: 19284291
Deposition date: 2008-09-09 Original release date: 2009-04-04
Authors: Link, Nina; Hunke, Cornelia; Eichler, Jutta; Bayer, Peter
Citation: Link, Nina; Hunke, Cornelia; Mueller, Jonathan; Eichler, Jutta; Bayer, Peter. "The solution structure of pGolemi, a high affinity Mena EVH1 binding miniature protein, suggests explanations for paralog-specific binding to Ena/VASP homology (EVH) 1 domains" Biol. Chem. 390, 417-26 (2009).
Assembly members:
pGolemi, polymer, 30 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
pGolemi: PFPPTPPGEEAPVEDLIRFY
NDLQQYLNVV
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 206 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | pGolemi | 1 |
Entities:
Entity 1, pGolemi 30 residues - Formula weight is not available
| 1 | PRO | PHE | PRO | PRO | THR | PRO | PRO | GLY | GLU | GLU | |
| 2 | ALA | PRO | VAL | GLU | ASP | LEU | ILE | ARG | PHE | TYR | |
| 3 | ASN | ASP | LEU | GLN | GLN | TYR | LEU | ASN | VAL | VAL |
Samples:
sample_1: pGolemi 1 mM; H2O 90%; D2O 10%; phosphate buffer 45 mM
sample_conditions_1: ionic strength: 45 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| COSY | sample_1 | isotropic | sample_conditions_1 |
| TOCSY | sample_1 | isotropic | sample_conditions_1 |
| NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
AUREMOL v2.0, Bruker Biospin - chemical shift assignment, peak picking
X-PLOR NIH v2.18, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution
NMR spectrometers:
- Bruker Avance 700 MHz
Related Database Links:
| PDB |