BMRB Entry 15981

Name: Solution NMR Structure of Cgi121 from Methanococcus jannaschii. Northeast Structural Genomics Consortium Target MJ0187
Published: 2009-02-09

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PDB ID: 2k8y
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15981
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR Structure of Cgi121 from Methanococcus jannaschii. Northeast Structural Genomics Consortium Target MJ0187

Deposition date:

2008-10-07

Original release date:

2009-02-09

Authors:

Rumpel, Sigrun; Fares, Christophe; Neculai, Dante; Arrowsmith, Cheryl; Sicheri, Frank

Citation:

Citation: Mao, Daniel; Neculai, Dante; Downey, Michael; Orlicky, Stephen; Haffani, Yosr; Ceccarelli, Derek; Ho, Jenny; Szilard, Rachel; Zhang, Wei; Ho, Cynthia; Wan, Leo; Fares, Christophe; Rumpel, Sigrun; Kurinov, Igor; Durocher, Daniel; Sicheri, Frank. "Atomic structure of the KEOPS complex: an ancient protein kinase-containing molecular machine" Mol. Cell 32, 259-275 (2008).
PubMed: 18951093

Assembly members:

Assembly members:
Cgi121, polymer, 150 residues, 17092.215 Da.

Natural source:

Natural source: Common Name: Methanococcus jannaschii Taxonomy ID: 243232 Superkingdom: Archaea Kingdom: Euryarchaeota Genus/species: Methanococcus jannaschii

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cgi121: GAMDPMIIRGIRGARINNEI FNLGLKFQILNADVVATKKH VLHAINQAKTKKPIAKSFWM EILVRASGQRQIHEAIKIIG AKDGNVCLICEDEETFRKIY ELIGGEIDDSVLEINEDKER LIREIFKIRGFGNVVERVLE KIALIELKKE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	700
15N chemical shifts	154
1H chemical shifts	1116

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Cgi121	1

Entities:

Entity 1, Cgi121 150 residues - 17092.215 Da.

1	GLY	ALA	MET	ASP	PRO	MET	ILE	ILE	ARG	GLY
2	ILE	ARG	GLY	ALA	ARG	ILE	ASN	ASN	GLU	ILE
3	PHE	ASN	LEU	GLY	LEU	LYS	PHE	GLN	ILE	LEU
4	ASN	ALA	ASP	VAL	VAL	ALA	THR	LYS	LYS	HIS
5	VAL	LEU	HIS	ALA	ILE	ASN	GLN	ALA	LYS	THR
6	LYS	LYS	PRO	ILE	ALA	LYS	SER	PHE	TRP	MET
7	GLU	ILE	LEU	VAL	ARG	ALA	SER	GLY	GLN	ARG
8	GLN	ILE	HIS	GLU	ALA	ILE	LYS	ILE	ILE	GLY
9	ALA	LYS	ASP	GLY	ASN	VAL	CYS	LEU	ILE	CYS
10	GLU	ASP	GLU	GLU	THR	PHE	ARG	LYS	ILE	TYR
11	GLU	LEU	ILE	GLY	GLY	GLU	ILE	ASP	ASP	SER
12	VAL	LEU	GLU	ILE	ASN	GLU	ASP	LYS	GLU	ARG
13	LEU	ILE	ARG	GLU	ILE	PHE	LYS	ILE	ARG	GLY
14	PHE	GLY	ASN	VAL	VAL	GLU	ARG	VAL	LEU	GLU
15	LYS	ILE	ALA	LEU	ILE	GLU	LEU	LYS	LYS	GLU

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
2D IPAP HSQC	sample_1	isotropic	sample_conditions_1
3D IPAP HNCO	sample_1	anisotropic	sample_conditions_2

PDB	2K8Y 3ENH
GB	AAB98167
SP	Q57646
AlphaFold	Q57646

BMRB Entry 15981

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	GLY	ALA	MET	ASP	PRO	MET	ILE	ILE	ARG	GLY
2	ILE	ARG	GLY	ALA	ARG	ILE	ASN	ASN	GLU	ILE
3	PHE	ASN	LEU	GLY	LEU	LYS	PHE	GLN	ILE	LEU
4	ASN	ALA	ASP	VAL	VAL	ALA	THR	LYS	LYS	HIS
5	VAL	LEU	HIS	ALA	ILE	ASN	GLN	ALA	LYS	THR
6	LYS	LYS	PRO	ILE	ALA	LYS	SER	PHE	TRP	MET
7	GLU	ILE	LEU	VAL	ARG	ALA	SER	GLY	GLN	ARG
8	GLN	ILE	HIS	GLU	ALA	ILE	LYS	ILE	ILE	GLY
9	ALA	LYS	ASP	GLY	ASN	VAL	CYS	LEU	ILE	CYS
10	GLU	ASP	GLU	GLU	THR	PHE	ARG	LYS	ILE	TYR
11	GLU	LEU	ILE	GLY	GLY	GLU	ILE	ASP	ASP	SER
12	VAL	LEU	GLU	ILE	ASN	GLU	ASP	LYS	GLU	ARG
13	LEU	ILE	ARG	GLU	ILE	PHE	LYS	ILE	ARG	GLY
14	PHE	GLY	ASN	VAL	VAL	GLU	ARG	VAL	LEU	GLU
15	LYS	ILE	ALA	LEU	ILE	GLU	LEU	LYS	LYS	GLU

1	GLY	ALA	MET	ASP	PRO	MET	ILE	ILE	ARG	GLY
2	ILE	ARG	GLY	ALA	ARG	ILE	ASN	ASN	GLU	ILE
3	PHE	ASN	LEU	GLY	LEU	LYS	PHE	GLN	ILE	LEU
4	ASN	ALA	ASP	VAL	VAL	ALA	THR	LYS	LYS	HIS
5	VAL	LEU	HIS	ALA	ILE	ASN	GLN	ALA	LYS	THR
6	LYS	LYS	PRO	ILE	ALA	LYS	SER	PHE	TRP	MET
7	GLU	ILE	LEU	VAL	ARG	ALA	SER	GLY	GLN	ARG
8	GLN	ILE	HIS	GLU	ALA	ILE	LYS	ILE	ILE	GLY
9	ALA	LYS	ASP	GLY	ASN	VAL	CYS	LEU	ILE	CYS
10	GLU	ASP	GLU	GLU	THR	PHE	ARG	LYS	ILE	TYR
11	GLU	LEU	ILE	GLY	GLY	GLU	ILE	ASP	ASP	SER
12	VAL	LEU	GLU	ILE	ASN	GLU	ASP	LYS	GLU	ARG
13	LEU	ILE	ARG	GLU	ILE	PHE	LYS	ILE	ARG	GLY
14	PHE	GLY	ASN	VAL	VAL	GLU	ARG	VAL	LEU	GLU
15	LYS	ILE	ALA	LEU	ILE	GLU	LEU	LYS	LYS	GLU

1	GLY	ALA	MET	ASP	PRO	MET	ILE	ILE	ARG	GLY
2	ILE	ARG	GLY	ALA	ARG	ILE	ASN	ASN	GLU	ILE
3	PHE	ASN	LEU	GLY	LEU	LYS	PHE	GLN	ILE	LEU
4	ASN	ALA	ASP	VAL	VAL	ALA	THR	LYS	LYS	HIS
5	VAL	LEU	HIS	ALA	ILE	ASN	GLN	ALA	LYS	THR
6	LYS	LYS	PRO	ILE	ALA	LYS	SER	PHE	TRP	MET
7	GLU	ILE	LEU	VAL	ARG	ALA	SER	GLY	GLN	ARG
8	GLN	ILE	HIS	GLU	ALA	ILE	LYS	ILE	ILE	GLY
9	ALA	LYS	ASP	GLY	ASN	VAL	CYS	LEU	ILE	CYS
10	GLU	ASP	GLU	GLU	THR	PHE	ARG	LYS	ILE	TYR
11	GLU	LEU	ILE	GLY	GLY	GLU	ILE	ASP	ASP	SER
12	VAL	LEU	GLU	ILE	ASN	GLU	ASP	LYS	GLU	ARG
13	LEU	ILE	ARG	GLU	ILE	PHE	LYS	ILE	ARG	GLY
14	PHE	GLY	ASN	VAL	VAL	GLU	ARG	VAL	LEU	GLU
15	LYS	ILE	ALA	LEU	ILE	GLU	LEU	LYS	LYS	GLU