BMRB Entry 16056

Name: STRUCTURAL AND FUNCTIONAL ANALYSIS OF TM XI OF THE NHE1 ISOFORM OF THE NA+/H+ EXCHANGER
Published: 2009-01-21

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PDB ID: 2kbv
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16056
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

STRUCTURAL AND FUNCTIONAL ANALYSIS OF TM XI OF THE NHE1 ISOFORM OF THE NA+/H+ EXCHANGER

Deposition date:

2008-12-09

Original release date:

2009-01-21

Authors:

Lee, Brian; Li, Xiuju; Liu, Yongsheng; Sykes, Brian; Fliegel, Larry

Citation:

Citation: Lee, Brian; Li, Xiuju; Liu, Yongsheng; Sykes, Brian; Fliegel, Larry. "Structural and Functional Analysis of TM XI of the NHE1 Isoform of the Na+/H+ Exchanger" J. Biol. Chem. 284, 11546-11556 (2009).
PubMed: 19176522

Assembly members:

Assembly members:
TMXI, polymer, 28 residues, 2860.407 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TMXI: XKDQFIIAYGGLRGAIAFSL GYLLDKKX

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	208
13C chemical shifts	46

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TMXI	1

Entities:

Entity 1, TMXI 28 residues - 2860.407 Da.

N terminal acetate cap, C terminal NH2 cap

1

ACE

LYS

ASP

GLN

PHE

ILE

ALA

TYR

GLY

2

GLY

LEU

ARG

GLY

ALA

ILE

ALA

PHE

SER

LEU

3

GLY

TYR

LEU

ASP

LYS

NH2

Samples:

sample_1: DPC 150 mM; DSS 0.25 mM; TMXI 2 mM; H2O 95%; D2O 5%

sample_conditions_1: temperature: 303 K; pH: 5; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
2D DQF-COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

X-PLOR_NIH v2.19, Schwieters, Kuszewski, Tjandra and Clore - structure solution, refinement

VNMRJ, Varian - collection

NMRView, Johnson, One Moon Scientific - peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Varian INOVA 800 MHz
Varian INOVA 500 MHz
Varian INOVA 600 MHz

PDB	2KBV
DBJ	BAE29332 BAE32882 BAE38221 BAG36869 BAG62158
EMBL	CAA10507 CAA42558 CAA43721 CAA69925
GB	AAA91483 AAA92976 AAA98479 AAB59460 AAC60606
REF	NP_001081553 NP_001095191 NP_001273957 NP_003038 NP_036784
SP	P19634 P23791 P26431 Q28036 Q61165
TPG	DAA32066
AlphaFold	P19634 P23791 P26431 Q28036 Q61165