BMRB Entry 16065
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PDB ID: 2kc8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16065
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Li, Guang-Yao; Zhang, Yonglong; Inouye, Masayori; Ikura, Mitsuhiko. "Inhibitory mechanism of E. coli RelE/RelB toxin/antitoxin module involves a
helix displacement near a mRNA interferase active site" J. Biol. Chem. 284, 14628-14636 (2009).
PubMed: 19297318
Assembly members:
RelE, polymer, 98 residues, 11336.2 Da.
RelBc, polymer, 36 residues, 4128.7 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
RelE: GSHMAYFLDFDERALKEWRK
LGSTVREQLKKKLVEVLESP
RIEANKLRGMPDCYKIKLRS
SGYRLVYQVIDEKVVVFVIS
VGKAEASEVYSEAVKRIL
RelBc: GSHKQTLLSDEDAELVEIVK
ERLRNPKPVRVTLDEL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 496 |
| 15N chemical shifts | 127 |
| 1H chemical shifts | 993 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | subunit A | 1 |
| 2 | subunit B | 2 |
Entities:
Entity 1, subunit A 98 residues - 11336.2 Da.
Residues 1-3 represent a non-native cloning artifact from the affinity tag.
| 1 | GLY | SER | HIS | MET | ALA | TYR | PHE | LEU | ASP | PHE | ||||
| 2 | ASP | GLU | ARG | ALA | LEU | LYS | GLU | TRP | ARG | LYS | ||||
| 3 | LEU | GLY | SER | THR | VAL | ARG | GLU | GLN | LEU | LYS | ||||
| 4 | LYS | LYS | LEU | VAL | GLU | VAL | LEU | GLU | SER | PRO | ||||
| 5 | ARG | ILE | GLU | ALA | ASN | LYS | LEU | ARG | GLY | MET | ||||
| 6 | PRO | ASP | CYS | TYR | LYS | ILE | LYS | LEU | ARG | SER | ||||
| 7 | SER | GLY | TYR | ARG | LEU | VAL | TYR | GLN | VAL | ILE | ||||
| 8 | ASP | GLU | LYS | VAL | VAL | VAL | PHE | VAL | ILE | SER | ||||
| 9 | VAL | GLY | LYS | ALA | GLU | ALA | SER | GLU | VAL | TYR | ||||
| 10 | SER | GLU | ALA | VAL | LYS | ARG | ILE | LEU |
Entity 2, subunit B 36 residues - 4128.7 Da.
Residues 1-3 represent a cloning artifact from the affinity tag.
| 1 | GLY | SER | HIS | LYS | GLN | THR | LEU | LEU | SER | ASP | ||||
| 2 | GLU | ASP | ALA | GLU | LEU | VAL | GLU | ILE | VAL | LYS | ||||
| 3 | GLU | ARG | LEU | ARG | ASN | PRO | LYS | PRO | VAL | ARG | ||||
| 4 | VAL | THR | LEU | ASP | GLU | LEU |
Related Database Links:
| BMRB | 16066 16067 |
| PDB | 2KC8 2KC9 3KIQ 3KIS 3KIU 3KIX 4FXE 4FXH 4FXI |
| DBJ | BAA15262 BAI25452 BAJ43363 BAA15263 BAI25453 BAJ43364 |
| EMBL | CAA26251 CBG34537 CCF87938 CDJ71963 CDP71409 CAA26250 CAQ98463 CBG34538 CBK86917 CCF87937 |
| GB | AAC74636 AAN43137 AAP17028 AAS76442 ABQ02944 AAC74637 AAN43138 AAP17029 ABF03723 ABP62831 |
| REF | NP_416081 NP_707430 WP_000323024 WP_000323025 WP_001513653 NP_416082 NP_707431 NP_837222 WP_000534858 WP_001413280 |
| SP | P0C077 P0C078 P0C079 P0C080 |
| AlphaFold | P0C077 P0C078 P0C079 P0C080 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks