BMRB Entry 16154

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PDB ID: 2ken
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16154
MolProbity Validation Chart

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Title:
Solution NMR structure of the OB domain (residues 67-166) of MM0293 from Methanosarcina mazei. Northeast Structural Genomics Consortium target MaR214a.
Deposition date:
2009-01-30
Original release date:
2009-03-05
Authors:
Ramelot, Theresa; Ding, Keyang; Magliqui, Melissa; Jiang, Mei; Ciccosanti, Colleen; Xiao, Rong; Lui, Jinfeng; Everett, John; Swapna, G.V.T.; Acton, Thomas; Rost, Burkhardt; Montelione, Gaetano; Kennedy, Michael
Citation:

Citation: Kennedy, Michael; Ramelot, Theresa; Ding, Keyang; Magliqui, Melissa; Jiang, Mei; Ciccosanti, Colleen; Xiao, Rong; Lui, Jinfeng; Everett, John; Swapna, G.V.T.; Acton, Thomas; Rost, Burkhardt; Montelione, Gaetano. "NMR structure of the OB domain of MM0293 from the archea Methanosarcina mazei"  .

Assembly members:

Assembly members:
OB-domain, polymer, 109 residues, 12519.227 Da.

Natural source:

Natural source:   Common Name: Methanosarcina mazei   Taxonomy ID: 2209   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanosarcina mazei

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21-23C

Entity Sequences (FASTA):

Entity Sequences (FASTA):
OB-domain: MEPQLTKIVDIVENGQWANL KAKVIQLWENTHESISQVGL LGDETGIIKFTIWKNAELPL LEQGESYLLRSVVVGEYNDR FQVQVNKNSSIEKLSEPIEV GLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts464
15N chemical shifts119
1H chemical shifts769

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1OB-domain1

Entities:

Entity 1, OB-domain 109 residues - 12519.227 Da.

Residues 2-100 correspond to residues 67-166 in the full-length protein. Residues 101-109 correspond to residues associated with the C-terminal His tag.

1   METGLUPROGLNLEUTHRLYSILEVALASP
2   ILEVALGLUASNGLYGLNTRPALAASNLEU
3   LYSALALYSVALILEGLNLEUTRPGLUASN
4   THRHISGLUSERILESERGLNVALGLYLEU
5   LEUGLYASPGLUTHRGLYILEILELYSPHE
6   THRILETRPLYSASNALAGLULEUPROLEU
7   LEUGLUGLNGLYGLUSERTYRLEULEUARG
8   SERVALVALVALGLYGLUTYRASNASPARG
9   PHEGLNVALGLNVALASNLYSASNSERSER
10   ILEGLULYSLEUSERGLUPROILEGLUVAL
11   GLYLEUGLUHISHISHISHISHISHIS

Related Database Links:

BMRB 16051
PDB 2KBN 2KEN

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