BMRB Entry 16339

Name: NMR structures of a designed Cyanovirin-N homolog lectin; LKAMG
Published: 2009-11-16

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PDB ID: 2kjl
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16339
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structures of a designed Cyanovirin-N homolog lectin; LKAMG

Deposition date:

2009-06-08

Original release date:

2009-11-16

Authors:

Koharudin, Leonardus; Gronenborn, Angela

Citation:

Citation: Koharudin, Leonardus; Furey, William; Gronenborn, Angela. "A designed chimeric cyanovirin-N homolog lectin: structure and molecular basis of sucrose binding." Proteins 77, 904-915 (2009).
PubMed: 19639634

Assembly members:

Assembly members:
LKAMG, polymer, 107 residues, 12104.285 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LKAMG: MSYADSSRNAVLTNGGRTLR AECRNADGNWVTSELDLDTI IGNNDGHFQWGGQNFTETAE DIRFHPKEGAAEQPILRARL RDCNGEFHDRDVNLNRIQNV NGRLVFQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	301
15N chemical shifts	121
1H chemical shifts	658

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	LKAMG	1

Entities:

Entity 1, LKAMG 107 residues - 12104.285 Da.

a triplet (GSH) artifact cloning is not included in the protein sequence and structure calculation.

1

MET

SER

TYR

ALA

ASP

SER

ARG

ASN

ALA

2

VAL

LEU

THR

ASN

GLY

ARG

THR

LEU

ARG

3

ALA

GLU

CYS

ARG

ASN

ALA

ASP

GLY

ASN

TRP

4

VAL

THR

SER

GLU

LEU

ASP

LEU

ASP

THR

ILE

5

ILE

GLY

ASN

ASP

GLY

HIS

PHE

GLN

TRP

6

GLY

GLN

ASN

PHE

THR

GLU

THR

ALA

GLU

7

ASP

ILE

ARG

PHE

HIS

PRO

LYS

GLU

GLY

ALA

8

ALA

GLU

GLN

PRO

ILE

LEU

ARG

ALA

ARG

LEU

9

ARG

ASP

CYS

ASN

GLY

GLU

PHE

HIS

ASP

ARG

10

ASP

VAL

ASN

LEU

ASN

ARG

ILE

GLN

ASN

VAL

11

ASN

GLY

ARG

LEU

VAL

PHE

GLN

Name	Sample	Sample state	Sample conditions
2D 15N-1H HSQC	sample_1	isotropic	sample_conditions_1
3D CBCANH	sample_2	isotropic	sample_conditions_1
3D CBCA(co)NH	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D H(c)CH-NH TOCSY	sample_2	isotropic	sample_conditions_1
(h)CCH-NH TOCSY	sample_2	isotropic	sample_conditions_1
3D HBHA(co)NH	sample_2	isotropic	sample_conditions_1
3D simultaneous 15N/13C NOESY-HSQC	sample_2	isotropic	sample_conditions_1

BMRB Entry 16339

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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