BMRB Entry 16364

Name: Solution NMR structure of FHA domain of Mb1858 from Mycobacterium bovis. Northeast Structural Genomics Consortium Target MbR243C (24-155).
Published: 2009-07-07

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PDB ID: 2kkl
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16364
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR structure of FHA domain of Mb1858 from Mycobacterium bovis. Northeast Structural Genomics Consortium Target MbR243C (24-155).

Deposition date:

2009-06-25

Original release date:

2009-07-07

Authors:

Yang, Yunhuang; Ramelot, Theresa; Wang, Dongyan; Foote, Erica; Jiang, M.; Nair, R.; Rost, Burkhard; Swapna, G.V.T; Acton, Thomas; Xiao, Rong; Everett, John; Montelio, Gaetano; Kennedy, Michael

Citation:

Citation: Yang, Yunhuang; Ramelot, Theresa; Wang, Dongyan; Foote, Erica; Jiang, M.; Nair, R.; Rost, Burkhard; Swapna, G.V.T; Acton, Thomas; Xiao, Rong; Everett, John; Montelio, Gaetano; Kennedy, Michael. "Solution NMR structure of FHA domain of Mb1858 from Mycobacterium bovis. Northeast Structural Genomics Consortium Target MbR243C (24-155)." .

Assembly members:

Assembly members:
Mb1858, polymer, 140 residues, 15241.8 Da.

Natural source:

Natural source: Common Name: Mycobacterium Bovis Taxonomy ID: 1765 Superkingdom: Bacteria Kingdom: not available Genus/species: Mycobacterium Bovis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 21-23C

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Mb1858: VFRADFLSELDAPAQAGTES AVSGVEGLPPGLALLVVKRG PNAGSRFLLDQAITSAGRHP DSDIFLDDVTVSRRHAEFRL ENNEFNVVDVGSLNGTYVNR EPVDSAVLANGDEVQIGKFR LVFLTGHKQGEDLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	586
15N chemical shifts	150
1H chemical shifts	941

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Mb1858	1

Entities:

Entity 1, Mb1858 140 residues - 15241.8 Da.

residues 24-155 of Mb1858 followed by 8 non-native C-terminal residues (LEHHHHHH)

1	VAL	PHE	ARG	ALA	ASP	PHE	LEU	SER	GLU	LEU
2	ASP	ALA	PRO	ALA	GLN	ALA	GLY	THR	GLU	SER
3	ALA	VAL	SER	GLY	VAL	GLU	GLY	LEU	PRO	PRO
4	GLY	LEU	ALA	LEU	LEU	VAL	VAL	LYS	ARG	GLY
5	PRO	ASN	ALA	GLY	SER	ARG	PHE	LEU	LEU	ASP
6	GLN	ALA	ILE	THR	SER	ALA	GLY	ARG	HIS	PRO
7	ASP	SER	ASP	ILE	PHE	LEU	ASP	ASP	VAL	THR
8	VAL	SER	ARG	ARG	HIS	ALA	GLU	PHE	ARG	LEU
9	GLU	ASN	ASN	GLU	PHE	ASN	VAL	VAL	ASP	VAL
10	GLY	SER	LEU	ASN	GLY	THR	TYR	VAL	ASN	ARG
11	GLU	PRO	VAL	ASP	SER	ALA	VAL	LEU	ALA	ASN
12	GLY	ASP	GLU	VAL	GLN	ILE	GLY	LYS	PHE	ARG
13	LEU	VAL	PHE	LEU	THR	GLY	HIS	LYS	GLN	GLY
14	GLU	ASP	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	NC_sample	isotropic	sample_conditions_1
2D 1H-13C HSQC	NC_sample	isotropic	sample_conditions_1
2D 1H-13C HSQC (no ct)	NC5_sample	isotropic	sample_conditions_1
3D 1H-15N NOESY	NC_sample	isotropic	sample_conditions_1
3D 1H-13C NOESY	NC_sample	isotropic	sample_conditions_1
3D HNCO	NC_sample	isotropic	sample_conditions_1
3D HNCA	NC_sample	isotropic	sample_conditions_1
3D HNCACB	NC_sample	isotropic	sample_conditions_1
3D CBCA(CO)NH	NC_sample	isotropic	sample_conditions_1
3D HN(CO)CA	NC_sample	isotropic	sample_conditions_1
3D HBHA(CO)NH	NC_sample	isotropic	sample_conditions_1
3D H(CCO)NH	NC_sample	isotropic	sample_conditions_1
3D C(CO)NH	NC_sample	isotropic	sample_conditions_1
3D HCCH-TOCSY	NC_sample	isotropic	sample_conditions_1
3D HCCH-COSY	NC_sample	isotropic	sample_conditions_1
3D CCH-TOCSY	NC_sample_in_D2O	isotropic	sample_conditions_1
4D CC NOESY	NC_sample_in_D2O	isotropic	sample_conditions_1
2D 1H-13C HSQC (ct)	NC5_sample	isotropic	sample_conditions_1
2D 1H-13C HSQC (arom)	NC5_sample	isotropic	sample_conditions_1
3D 1H-13C NOESY (arom)	NC_sample	isotropic	sample_conditions_1

BMRB	16248
PDB	2KFU 2KKL
DBJ	BAH26133 BAL65810 BAQ05852 BAQ05855 GAA45536
EMBL	CAL71849 CCC26919 CCC44174 CCC64431 CCE37310
GB	AAK46148 ABQ73594 ABR06192 ACT25235 AEB04307
REF	NP_216343 NP_855510 WP_003899041 WP_015303233 WP_021083142
SP	P64898 P9WJA8 P9WJA9
AlphaFold	P64898 P9WJA8 P9WJA9

BMRB Entry 16364

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	VAL	PHE	ARG	ALA	ASP	PHE	LEU	SER	GLU	LEU
2	ASP	ALA	PRO	ALA	GLN	ALA	GLY	THR	GLU	SER
3	ALA	VAL	SER	GLY	VAL	GLU	GLY	LEU	PRO	PRO
4	GLY	LEU	ALA	LEU	LEU	VAL	VAL	LYS	ARG	GLY
5	PRO	ASN	ALA	GLY	SER	ARG	PHE	LEU	LEU	ASP
6	GLN	ALA	ILE	THR	SER	ALA	GLY	ARG	HIS	PRO
7	ASP	SER	ASP	ILE	PHE	LEU	ASP	ASP	VAL	THR
8	VAL	SER	ARG	ARG	HIS	ALA	GLU	PHE	ARG	LEU
9	GLU	ASN	ASN	GLU	PHE	ASN	VAL	VAL	ASP	VAL
10	GLY	SER	LEU	ASN	GLY	THR	TYR	VAL	ASN	ARG
11	GLU	PRO	VAL	ASP	SER	ALA	VAL	LEU	ALA	ASN
12	GLY	ASP	GLU	VAL	GLN	ILE	GLY	LYS	PHE	ARG
13	LEU	VAL	PHE	LEU	THR	GLY	HIS	LYS	GLN	GLY
14	GLU	ASP	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

1	VAL	PHE	ARG	ALA	ASP	PHE	LEU	SER	GLU	LEU
2	ASP	ALA	PRO	ALA	GLN	ALA	GLY	THR	GLU	SER
3	ALA	VAL	SER	GLY	VAL	GLU	GLY	LEU	PRO	PRO
4	GLY	LEU	ALA	LEU	LEU	VAL	VAL	LYS	ARG	GLY
5	PRO	ASN	ALA	GLY	SER	ARG	PHE	LEU	LEU	ASP
6	GLN	ALA	ILE	THR	SER	ALA	GLY	ARG	HIS	PRO
7	ASP	SER	ASP	ILE	PHE	LEU	ASP	ASP	VAL	THR
8	VAL	SER	ARG	ARG	HIS	ALA	GLU	PHE	ARG	LEU
9	GLU	ASN	ASN	GLU	PHE	ASN	VAL	VAL	ASP	VAL
10	GLY	SER	LEU	ASN	GLY	THR	TYR	VAL	ASN	ARG
11	GLU	PRO	VAL	ASP	SER	ALA	VAL	LEU	ALA	ASN
12	GLY	ASP	GLU	VAL	GLN	ILE	GLY	LYS	PHE	ARG
13	LEU	VAL	PHE	LEU	THR	GLY	HIS	LYS	GLN	GLY
14	GLU	ASP	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

1	VAL	PHE	ARG	ALA	ASP	PHE	LEU	SER	GLU	LEU
2	ASP	ALA	PRO	ALA	GLN	ALA	GLY	THR	GLU	SER
3	ALA	VAL	SER	GLY	VAL	GLU	GLY	LEU	PRO	PRO
4	GLY	LEU	ALA	LEU	LEU	VAL	VAL	LYS	ARG	GLY
5	PRO	ASN	ALA	GLY	SER	ARG	PHE	LEU	LEU	ASP
6	GLN	ALA	ILE	THR	SER	ALA	GLY	ARG	HIS	PRO
7	ASP	SER	ASP	ILE	PHE	LEU	ASP	ASP	VAL	THR
8	VAL	SER	ARG	ARG	HIS	ALA	GLU	PHE	ARG	LEU
9	GLU	ASN	ASN	GLU	PHE	ASN	VAL	VAL	ASP	VAL
10	GLY	SER	LEU	ASN	GLY	THR	TYR	VAL	ASN	ARG
11	GLU	PRO	VAL	ASP	SER	ALA	VAL	LEU	ALA	ASN
12	GLY	ASP	GLU	VAL	GLN	ILE	GLY	LYS	PHE	ARG
13	LEU	VAL	PHE	LEU	THR	GLY	HIS	LYS	GLN	GLY
14	GLU	ASP	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS