BMRB Entry 16400

Assembly:

Entities:

Entity 1, collagen A 30 residues - 2849.519 Da.

Entity 2, collagen B 30 residues - 2868.582 Da.

Entity 3, collagen C 30 residues - 2688.862 Da.

Samples:

sample_1: entity_1 1.2 mM; H2O 90%; D2O 10%

sample_2: entity_2 1.2 mM; H2O 90%; D2O 10%

sample_3: entity_3 1.2 mM; H2O 90%; D2O 10%

sample_4: entity_1 1.2 mM; entity_2 1.2 mM; entity_3 1.2 mM; H2O 90%; D2O 10%

sample_5: entity_1, [U-99% 13C; U-99% 15N], 1.2 mM; entity_2, [U-99% 13C; U-99% 15N], 1.2 mM; entity_3, [U-99% 13C; U-99% 15N], 1.2 mM; H2O 90%; D2O 10%; phosphate buffer 10 mM

sample_6: entity_1 1.8 mM; entity_2 1.2 mM; entity_3 1.2 mM; H2O 90%; D2O 10%; phosphate buffer 10 mM

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

sample_conditions_2: pH: 7; pressure: 1 atm; temperature: 283 K

Experiments:

Software:

CNS vv 1.21, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

SPARKY, Goddard - chemical shift assignment

AMBER v9, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

CcpNMR, CCPN - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

• Varian INOVA 800 MHz