BMRB Entry 16425

Name: Interdomain RRM packing contributes to RNA recognition in the rna15, hrp1, anchor RNA 3&#039; processing ternary complex
Published: 2010-07-27

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PDB ID: 2km8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16425
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Interdomain RRM packing contributes to RNA recognition in the rna15, hrp1, anchor RNA 3' processing ternary complex

Deposition date:

2009-07-24

Original release date:

2010-07-27

Authors:

Leeper, Thomas; Varani, Gabriele

Citation:

Citation: Leeper, Thomas; Qu, Xiangping; Lu, Connie; Moore, Claire; Varani, Gabriele. "Novel Protein-Protein Contacts Facilitate mRNA 3'-Processing Signal Recognition by Rna15 and Hrp1." J. Mol. Biol. 401, 334-349 (2010).
PubMed: 20600122

Assembly members:

Assembly members:
rna15p, polymer, 84 residues, Formula weight is not available
anchor_RNA, polymer, 13 residues, 112.088 Da.
hrp1p, polymer, 167 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet32

Entity Sequences (FASTA):

Entity Sequences (FASTA):
rna15p: NPPSRVVYLGSIPYDQTEEQ ILDLCSNVGPVINLKMMFDP QTGRSKGYAFIEFRDLESSA SAVRNLNGYQLGSRFLKCGY SSNS
anchor_RNA: UAUAUAUAAUAAU
hrp1p: KESCKMFIGGLNWDTTEDNL REYFGKYGTVTDLKIMKDPA TGRSRGFGFLSFEKPSSVDE VVKTQHILDGKVIDPKRAIP RDEQDKTGKIFVGGIGPDVR PKEFEEFFSQWGTIIDAQLM LDKDTGQSRGFGFVTYDSAD AVDRVCQNKFIDFKDRKIEI KRAEPRH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	280
15N chemical shifts	88
1H chemical shifts	554

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	rna15p	1
2	anchor_RNA	2
3	hrp1p	3

Entities:

Entity 1, rna15p 84 residues - Formula weight is not available

1

ASN

PRO

SER

ARG

VAL

TYR

LEU

GLY

2

SER

ILE

PRO

TYR

ASP

GLN

THR

GLU

GLN

3

ILE

LEU

ASP

LEU

CYS

SER

ASN

VAL

GLY

PRO

4

VAL

ILE

ASN

LEU

LYS

MET

PHE

ASP

PRO

5

GLN

THR

GLY

ARG

SER

LYS

GLY

TYR

ALA

PHE

6

ILE

GLU

PHE

ARG

ASP

LEU

GLU

SER

ALA

7

SER

ALA

VAL

ARG

ASN

LEU

ASN

GLY

TYR

GLN

8

LEU

GLY

SER

ARG

PHE

LEU

LYS

CYS

GLY

TYR

9

SER

ASN

SER

Entity 2, anchor_RNA 13 residues - 112.088 Da.

1

U

A

U

A

U

A

U

A

U

2

A

U

Entity 3, hrp1p 167 residues - Formula weight is not available

1

LYS

GLU

SER

CYS

LYS

MET

PHE

ILE

GLY

2

LEU

ASN

TRP

ASP

THR

GLU

ASP

ASN

LEU

3

ARG

GLU

TYR

PHE

GLY

LYS

TYR

GLY

THR

VAL

4

THR

ASP

LEU

LYS

ILE

MET

LYS

ASP

PRO

ALA

5

THR

GLY

ARG

SER

ARG

GLY

PHE

GLY

PHE

LEU

6

SER

PHE

GLU

LYS

PRO

SER

VAL

ASP

GLU

7

VAL

LYS

THR

GLN

HIS

ILE

LEU

ASP

GLY

8

LYS

VAL

ILE

ASP

PRO

LYS

ARG

ALA

ILE

PRO

9

ARG

ASP

GLU

GLN

ASP

LYS

THR

GLY

LYS

ILE

10

PHE

VAL

GLY

ILE

GLY

PRO

ASP

VAL

ARG

11

PRO

LYS

GLU

PHE

GLU

PHE

SER

GLN

12

TRP

GLY

THR

ILE

ASP

ALA

GLN

LEU

MET

13

LEU

ASP

LYS

ASP

THR

GLY

GLN

SER

ARG

GLY

14

PHE

GLY

PHE

VAL

THR

TYR

ASP

SER

ALA

ASP

15

ALA

VAL

ASP

ARG

VAL

CYS

GLN

ASN

LYS

PHE

16

ILE

ASP

PHE

LYS

ASP

ARG

LYS

ILE

GLU

ILE

17

LYS

ARG

ALA

GLU

PRO

ARG

HIS

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_3	anisotropic	sample_conditions_1
2D 1H-15N HSQC	sample_4	anisotropic	sample_conditions_1
2D 1H-13C HSQC	sample_3	anisotropic	sample_conditions_1
2D 1H-13C HSQC	sample_4	anisotropic	sample_conditions_1
2D 1H-13C HSQC	sample_5	isotropic	sample_conditions_1
3D HNCO	sample_5	isotropic	sample_conditions_1
3D HNCA	sample_5	isotropic	sample_conditions_1
3D HNCACB	sample_5	isotropic	sample_conditions_1
3D HN(CO)CA	sample_5	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_5	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_5	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_5	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_5	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_5	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_6	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_6	isotropic	sample_conditions_1

PDB	2KM8 2X1A 2X1B 2X1F 2CJK 2KM8
DBJ	GAA23344 GAA26207
EMBL	CAA96746 CAY79715 CAA64546 CAA99142 CAY86168
GB	AAA34984 AHY79328 AJP38746 AJR76066 AJR76567 AAA79097 AAB18142 AHY77195 EDN63753 EDV10496
REF	NP_011471 NP_014518 XP_011105369
SP	P25299 Q99383
TPG	DAA08056 DAA10661
AlphaFold	P25299 Q99383

BMRB Entry 16425

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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