BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16504

Title: Human cannabinoid receptor 1 - helix 7/8 peptide   PubMed: 19766594

Deposition date: 2009-09-18 Original release date: 2009-11-20

Authors: Deshmukh, Lalit; Vinogradova, Olga; Makriyannis, Alexandros; Tiburu, Elvis; Tyukhtenko, Sergiy; Janero, David

Citation: Tyukhtenko, Sergiy; Tiburu, Elvis; Deshmukh, Lalit; Vinogradova, Olga; Janero, David; Makriyannis, Alexandros. "NMR solution structure of human cannabinoid receptor-1 helix 7/8 peptide: candidate electrostatic interactions and microdomain formation."  Biochem. Biophys. Res. Commun. 390, 441-446 (2009).

Assembly members:
Human cannabinoid receptor 1, polymer, 40 residues, 4524.348 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Human cannabinoid receptor 1: TVFAFASMLCLLNSTVNPII YALRSKDLRHAFRSMFPSAE

Data sets:
Data typeCount
1H chemical shifts260

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Human cannabinoid receptor 11

Entities:

Entity 1, Human cannabinoid receptor 1 40 residues - 4524.348 Da.

1   THRVALPHEALAPHEALASERMETLEUCYS
2   LEULEUASNSERTHRVALASNPROILEILE
3   TYRALALEUARGSERLYSASPLEUARGHIS
4   ALAPHEARGSERMETPHEPROSERALAGLU

Samples:

sample_1: entity 1 mM; H2O 70%; TFE 30%

sample_conditions_1: pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

AUREMOL, Bruker Biospin - data analysis

X-PLOR NIH v2.21, Schwieters, Kuszewski, Tjandra and Clore - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PDB
EMBL CDQ70034
GB AAY21179 EGV93809
REF XP_013999659