BMRB Entry 16520

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PDB ID: 2kom
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16520
MolProbity Validation Chart

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Title:
Solution structure of humar Par-3b PDZ2 (residues 451-549)
Deposition date:
2009-09-24
Original release date:
2010-01-28
Authors:
Volkman, B.; Tyler, R.; Peterson, F.
Citation:

Citation: Jensen, Davin; Woytovich, Christopher; Li, Margie; Duvnjak, Petar; Cassidy, Michael; Frederick, Ronnie; Bergeman, Lai; Peterson, Francis; Volkman, Brian. "Rapid, robotic, small-scale protein production for NMR screening and structure determination."  Protein Sci. 19, 570-578 (2010).
PubMed: 20073081

Assembly members:

Assembly members:
hPar-3_PDZ2, polymer, 121 residues, 13394.356 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo Sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE30-8HT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hPar-3_PDZ2: MRGSHHHHHHHHGSENLYFQ GSYNTKKIGKRLNIQLKKGT EGLGFSITSRDVTIGGSAPI YVKNILPRGAAIQDGRLKAG DRLIEVNGVDLVGKSQEEVV SLLRSTKMEGTVSLLVFRQE D

Data sets:
Data typeCount
13C chemical shifts393
15N chemical shifts99
1H chemical shifts616

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1hPar3 PDZ21

Entities:

Entity 1, hPar3 PDZ2 121 residues - 13394.356 Da.

Residues 429-450 (MRGSHHHHHHHHGSENLYFQGS) are a octa-His tag that was not removed from the protein prior to structure determination.

1   METARGGLYSERHISHISHISHISHISHIS
2   HISHISGLYSERGLUASNLEUTYRPHEGLN
3   GLYSERTYRASNTHRLYSLYSILEGLYLYS
4   ARGLEUASNILEGLNLEULYSLYSGLYTHR
5   GLUGLYLEUGLYPHESERILETHRSERARG
6   ASPVALTHRILEGLYGLYSERALAPROILE
7   TYRVALLYSASNILELEUPROARGGLYALA
8   ALAILEGLNASPGLYARGLEULYSALAGLY
9   ASPARGLEUILEGLUVALASNGLYVALASP
10   LEUVALGLYLYSSERGLNGLUGLUVALVAL
11   SERLEULEUARGSERTHRLYSMETGLUGLY
12   THRVALSERLEULEUVALPHEARGGLNGLU
13   ASP

Related Database Links:

BMRB 16515
PDB 2KOJ 2KOM
GB EPQ12192
REF XP_011836158

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks