BMRB Entry 16638

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PDB ID: 2krg
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16638
MolProbity Validation Chart

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Title:
Solution Structure of human sodium/ hydrogen exchange regulatory factor 1(150-358).
Deposition date:
2009-12-18
Original release date:
2010-02-01
Authors:
Bhattacharya, Shibani; Dai, Zhongping; Li, Jianquan; Baxter, Sabine; Callaway, David; Cowburn, David; Bu, Zimei
Citation:

Citation: Bhattacharya, Shibani; Dai, Zhongping; Li, Jianquan; Baxter, Sabine; Callaway, David; Cowburn, David; Bu, Zimei. "A conformational switch in the scaffolding protein NHERF1 controls autoinhibition and complex formation."  J. Biol. Chem. 285, 9981-9994 (2010).
PubMed: 20042604

Assembly members:

Assembly members:
NHERF1_(150-358), polymer, 216 residues, 23669.648 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET151/D-TOPO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NHERF1_(150-358): GIDPFTMLRPRLCTMKKGPS GYGFNLHSDKSKPGQFIRSV DPDSPAEASGLRAQDRIVEV NGVCMEGKQHGDVVSAIRAG GDETKLLVVDRETDEFFKKC RVIPSQEHLNGPLPVPFTNG EIQKENSREALAEAALESPR PALVRSASSDTSEELNSQDS PPKQDSTAPSSTSSSDPILD FNISLAMAKERAHQKRSSKR APQMDWSKKNELFSNL

Data sets:
Data typeCount
13C chemical shifts758
15N chemical shifts174
1H chemical shifts1241

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1NHERF1_(150-358)1

Entities:

Entity 1, NHERF1_(150-358) 216 residues - 23669.648 Da.

Residues 1-7 represent a non-native affinity tag

1   GLYILEASPPROPHETHRMETLEUARGPRO
2   ARGLEUCYSTHRMETLYSLYSGLYPROSER
3   GLYTYRGLYPHEASNLEUHISSERASPLYS
4   SERLYSPROGLYGLNPHEILEARGSERVAL
5   ASPPROASPSERPROALAGLUALASERGLY
6   LEUARGALAGLNASPARGILEVALGLUVAL
7   ASNGLYVALCYSMETGLUGLYLYSGLNHIS
8   GLYASPVALVALSERALAILEARGALAGLY
9   GLYASPGLUTHRLYSLEULEUVALVALASP
10   ARGGLUTHRASPGLUPHEPHELYSLYSCYS
11   ARGVALILEPROSERGLNGLUHISLEUASN
12   GLYPROLEUPROVALPROPHETHRASNGLY
13   GLUILEGLNLYSGLUASNSERARGGLUALA
14   LEUALAGLUALAALALEUGLUSERPROARG
15   PROALALEUVALARGSERALASERSERASP
16   THRSERGLUGLULEUASNSERGLNASPSER
17   PROPROLYSGLNASPSERTHRALAPROSER
18   SERTHRSERSERSERASPPROILELEUASP
19   PHEASNILESERLEUALAMETALALYSGLU
20   ARGALAHISGLNLYSARGSERSERLYSARG
21   ALAPROGLNMETASPTRPSERLYSLYSASN
22   GLULEUPHESERASNLEU

Related Database Links:

BMRB 16637 18826
PDB 2KJD 2KRG 2M0V
DBJ BAG54683
GB EAW89189 EHH58322

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