BMRB Entry 16681

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PDB ID: 2kt1
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16681
MolProbity Validation Chart

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Title:
Solution NMR Structure of the SH3 Domain from the p85beta subunit of Phosphatidylinositol 3-kinase from H.sapiens. Northeast Structural Genomics Consortium Target HR5531E.
Deposition date:
2010-01-15
Original release date:
2010-02-22
Authors:
Aramini, James; Ma, Li-Chung; Schauder, Curtis; Everett, John; Montelione, Gaetano
Citation:

Citation: Aramini, James; Ma, Li-Chung; Schauder, Curtis; Everett, John; Montelione, Gaetano. "Solution NMR Structure of the SH3 Domain from the p85beta subunit of Phosphatidylinositol 3-kinase from H.sapiens. Northeast Structural Genomics Consortium Target HR5531E."  To be published ., .-..

Assembly members:

Assembly members:
HR5531E, polymer, 88 residues, 9130.386 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: HR5531E-1-80-21.1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HR5531E: MAGPEGFQYRALYPFRRERP EDLELLPGDVLVVSRAALQA LGVAEGGERCPQSVGWMPGL NERTRQRGDFPGTYVEFLGP LEHHHHHH

Data typeCount
13C chemical shifts350
15N chemical shifts73
1H chemical shifts543

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1HR5531E1

Entities:

Entity 1, HR5531E 88 residues - 9130.386 Da.

Contains additional C-terminal LEHHHHHH affinity tag

1   METALAGLYPROGLUGLYPHEGLNTYRARG
2   ALALEUTYRPROPHEARGARGGLUARGPRO
3   GLUASPLEUGLULEULEUPROGLYASPVAL
4   LEUVALVALSERARGALAALALEUGLNALA
5   LEUGLYVALALAGLUGLYGLYGLUARGCYS
6   PROGLNSERVALGLYTRPMETPROGLYLEU
7   ASNGLUARGTHRARGGLNARGGLYASPPHE
8   PROGLYTHRTYRVALGLUPHELEUGLYPRO
9   LEUGLUHISHISHISHISHISHIS

Related Database Links:

PDB 2KT1 3O5Z
DBJ BAG11075
EMBL CAA56868
GB AAA79510 AAD22671 AAH32647 AAH70082 AAH90249
REF NP_001244981 NP_005018 NP_777001 XP_002761945 XP_003275871
SP O00459 P23726
TPG DAA28159
AlphaFold O00459 P23726

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks