BMRB Entry 16734

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PDB ID: 2kuf
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16734
MolProbity Validation Chart

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Title:
NMR structure of the PASTA domain 3 and 4 of Mycobacterium tuberculosis of PknB
Deposition date:
2010-02-17
Original release date:
2010-05-18
Authors:
Barthe, Philippe; Cohen-Gonsaud, Martin; Roumestand, Christian; Mukamolova, Galina
Citation:

Citation: Barthe, Philippe; Mukamolova, Galina; Roumestand, Christian; Cohen-Gonsaud, Martin. "The structure of PknB extracellular PASTA domain from mycobacterium tuberculosis suggests a ligand-dependent kinase activation."  Structure (Cambridge, MA, U. S.) 18, 606-615 (2010).
PubMed: 20462494

Assembly members:

Assembly members:
PknB_PASTA34, polymer, 139 residues, 14203.956 Da.

Natural source:

Natural source:   Common Name: Mycobacterium tuberculosis   Taxonomy ID: 1773   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Mycobacterium tuberculosis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21-TEV

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PknB_PASTA34: GHMGPATKDIPDVAGQTVDV AQKNLNVYGFTKFSQASVDS PRPAGEVTGTNPPAGTTVPV DSVIELQVSKGNQFVMPDLS GMFWVDAEPRLRALGWTGML DKGADVDAGGSQHNRVVYQN PPAGTGVNRDGIITLRFGQ

Data sets:
Data typeCount
13C chemical shifts378
15N chemical shifts148
1H chemical shifts890

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 139 residues - 14203.956 Da.

1   GLYHISMETGLYPROALATHRLYSASPILE
2   PROASPVALALAGLYGLNTHRVALASPVAL
3   ALAGLNLYSASNLEUASNVALTYRGLYPHE
4   THRLYSPHESERGLNALASERVALASPSER
5   PROARGPROALAGLYGLUVALTHRGLYTHR
6   ASNPROPROALAGLYTHRTHRVALPROVAL
7   ASPSERVALILEGLULEUGLNVALSERLYS
8   GLYASNGLNPHEVALMETPROASPLEUSER
9   GLYMETPHETRPVALASPALAGLUPROARG
10   LEUARGALALEUGLYTRPTHRGLYMETLEU
11   ASPLYSGLYALAASPVALASPALAGLYGLY
12   SERGLNHISASNARGVALVALTYRGLNASN
13   PROPROALAGLYTHRGLYVALASNARGASP
14   GLYILEILETHRLEUARGPHEGLYGLN

Related Database Links:

PDB 2KUF 3OUV

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