BMRB Entry 16789

Name: Solution NMR structure of human ubiquitin specific protease Usp7 UBL domain (residues 537-664). NESG target hr4395c/ SGC-Toronto
Published: 2010-09-24

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PDB ID: 2kvr
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16789
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR structure of human ubiquitin specific protease Usp7 UBL domain (residues 537-664). NESG target hr4395c/ SGC-Toronto

Deposition date:

2010-03-25

Original release date:

2010-09-24

Authors:

Bezsonova, Irina; Lemak, Alexander; Avvakumov, George; Bezsonova, Irina; Dhe-Paganon, Sirano; Arrowsmith, Cheryl

Citation:

Citation: Bezsonova, Irina; Avvakumov, George; Dhe-Paganon, Sirano; Arrowsmith, Cheryl. "solution structure of the ubiquitin specific protease Usp7 ubiquitin-like domain" .

Assembly members:

Assembly members:
usp7-ubl1, polymer, 130 residues, 15040.011 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
usp7-ubl1: GSPQQLVERLQEEKRIEAQK RKERQEAHLYMQVQIVAEDQ FCGHQGNDMYDEEKVKYTVF KVLKNSSLAEFVQSLSQTMG FPQDQIRLWPMQARSNGTKR PAMLDNEADGNKTMIELSDN ENPWTIFLET

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	524
15N chemical shifts	123
1H chemical shifts	866

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	usp7-ubl1	1

Entities:

Entity 1, usp7-ubl1 130 residues - 15040.011 Da.

residues 1-2 are non-native. Residues 3-130 correspond to residues 537-664 in the full length Usp7.

1	GLY	SER	PRO	GLN	GLN	LEU	VAL	GLU	ARG	LEU
2	GLN	GLU	GLU	LYS	ARG	ILE	GLU	ALA	GLN	LYS
3	ARG	LYS	GLU	ARG	GLN	GLU	ALA	HIS	LEU	TYR
4	MET	GLN	VAL	GLN	ILE	VAL	ALA	GLU	ASP	GLN
5	PHE	CYS	GLY	HIS	GLN	GLY	ASN	ASP	MET	TYR
6	ASP	GLU	GLU	LYS	VAL	LYS	TYR	THR	VAL	PHE
7	LYS	VAL	LEU	LYS	ASN	SER	SER	LEU	ALA	GLU
8	PHE	VAL	GLN	SER	LEU	SER	GLN	THR	MET	GLY
9	PHE	PRO	GLN	ASP	GLN	ILE	ARG	LEU	TRP	PRO
10	MET	GLN	ALA	ARG	SER	ASN	GLY	THR	LYS	ARG
11	PRO	ALA	MET	LEU	ASP	ASN	GLU	ALA	ASP	GLY
12	ASN	LYS	THR	MET	ILE	GLU	LEU	SER	ASP	ASN
13	GLU	ASN	PRO	TRP	THR	ILE	PHE	LEU	GLU	THR

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D aromatic 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

PDB	2KVR 2YLM 4PYZ 4WPH 4YOC 5C56 5C6D
DBJ	BAH13721 BAH13801 BAH13841 BAH14812
EMBL	CAA96580
GB	AAI00667 AAI66012 AAI66690 AAQ12339 AAQ74887
REF	NP_001003918 NP_001019961 NP_001129152 NP_001273386 NP_001273387
SP	Q4VSI4 Q6A4J8 Q6U7I1 Q93009
AlphaFold	Q4VSI4 Q6A4J8 Q6U7I1 Q93009

BMRB Entry 16789

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	GLY	SER	PRO	GLN	GLN	LEU	VAL	GLU	ARG	LEU
2	GLN	GLU	GLU	LYS	ARG	ILE	GLU	ALA	GLN	LYS
3	ARG	LYS	GLU	ARG	GLN	GLU	ALA	HIS	LEU	TYR
4	MET	GLN	VAL	GLN	ILE	VAL	ALA	GLU	ASP	GLN
5	PHE	CYS	GLY	HIS	GLN	GLY	ASN	ASP	MET	TYR
6	ASP	GLU	GLU	LYS	VAL	LYS	TYR	THR	VAL	PHE
7	LYS	VAL	LEU	LYS	ASN	SER	SER	LEU	ALA	GLU
8	PHE	VAL	GLN	SER	LEU	SER	GLN	THR	MET	GLY
9	PHE	PRO	GLN	ASP	GLN	ILE	ARG	LEU	TRP	PRO
10	MET	GLN	ALA	ARG	SER	ASN	GLY	THR	LYS	ARG
11	PRO	ALA	MET	LEU	ASP	ASN	GLU	ALA	ASP	GLY
12	ASN	LYS	THR	MET	ILE	GLU	LEU	SER	ASP	ASN
13	GLU	ASN	PRO	TRP	THR	ILE	PHE	LEU	GLU	THR

1	GLY	SER	PRO	GLN	GLN	LEU	VAL	GLU	ARG	LEU
2	GLN	GLU	GLU	LYS	ARG	ILE	GLU	ALA	GLN	LYS
3	ARG	LYS	GLU	ARG	GLN	GLU	ALA	HIS	LEU	TYR
4	MET	GLN	VAL	GLN	ILE	VAL	ALA	GLU	ASP	GLN
5	PHE	CYS	GLY	HIS	GLN	GLY	ASN	ASP	MET	TYR
6	ASP	GLU	GLU	LYS	VAL	LYS	TYR	THR	VAL	PHE
7	LYS	VAL	LEU	LYS	ASN	SER	SER	LEU	ALA	GLU
8	PHE	VAL	GLN	SER	LEU	SER	GLN	THR	MET	GLY
9	PHE	PRO	GLN	ASP	GLN	ILE	ARG	LEU	TRP	PRO
10	MET	GLN	ALA	ARG	SER	ASN	GLY	THR	LYS	ARG
11	PRO	ALA	MET	LEU	ASP	ASN	GLU	ALA	ASP	GLY
12	ASN	LYS	THR	MET	ILE	GLU	LEU	SER	ASP	ASN
13	GLU	ASN	PRO	TRP	THR	ILE	PHE	LEU	GLU	THR

1	GLY	SER	PRO	GLN	GLN	LEU	VAL	GLU	ARG	LEU
2	GLN	GLU	GLU	LYS	ARG	ILE	GLU	ALA	GLN	LYS
3	ARG	LYS	GLU	ARG	GLN	GLU	ALA	HIS	LEU	TYR
4	MET	GLN	VAL	GLN	ILE	VAL	ALA	GLU	ASP	GLN
5	PHE	CYS	GLY	HIS	GLN	GLY	ASN	ASP	MET	TYR
6	ASP	GLU	GLU	LYS	VAL	LYS	TYR	THR	VAL	PHE
7	LYS	VAL	LEU	LYS	ASN	SER	SER	LEU	ALA	GLU
8	PHE	VAL	GLN	SER	LEU	SER	GLN	THR	MET	GLY
9	PHE	PRO	GLN	ASP	GLN	ILE	ARG	LEU	TRP	PRO
10	MET	GLN	ALA	ARG	SER	ASN	GLY	THR	LYS	ARG
11	PRO	ALA	MET	LEU	ASP	ASN	GLU	ALA	ASP	GLY
12	ASN	LYS	THR	MET	ILE	GLU	LEU	SER	ASP	ASN
13	GLU	ASN	PRO	TRP	THR	ILE	PHE	LEU	GLU	THR