BMRB Entry 16812

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PDB ID: 2kae
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16812
MolProbity Validation Chart

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Title:
data-driven model of MED1:DNA complex
Deposition date:
2010-03-31
Original release date:
2010-04-29
Authors:
Lowry, Jason; Gamsjaeger, Roland; Thong, Sock Yue; Hung, Wendy; Kwan, Ann; Broitman-Maduro, Gina; Matthews, Jacqueline; Maduro, Morris; Mackay, Joel
Citation:

Citation: Lowry, Jason; Gamsjaeger, Roland; Thong, Sock Yue; Hung, Wendy; Kwan, Ann; Broitman-Maduro, Gina; Matthews, Jacqueline; Maduro, Morris; Mackay, Joel. "Structural analysis of MED-1 reveals unexpected diversity in the mechanism of DNA recognition by GATA-type zinc finger domains."  J. Biol. Chem. 284, 5827-5835 (2009).
PubMed: 19095651

Assembly members:

Assembly members:
MED1zf, polymer, 65 residues, 6728.883 Da.
ZN, non-polymer, 65.409 Da.
DNA, polymer, . residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Caenorhabditis elegans   Taxonomy ID: 6239   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Caenorhabditis elegans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MED1zf: KSFQCSNCSVTETIRWRNIR SKEGIQCNACFIYQRKYNKT RPVTAVNKYQKRKLKVQETN GVDSF
DNA: CGGAAAAGTATACTTTTCCG

Data sets:
Data typeCount
13C chemical shifts145
15N chemical shifts65
1H chemical shifts299

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MED1zf1
2ZINC ION2
3DNA3

Entities:

Entity 1, MED1zf 65 residues - 6728.883 Da.

1   LYSSERPHEGLNCYSSERASNCYSSERVAL
2   THRGLUTHRILEARGTRPARGASNILEARG
3   SERLYSGLUGLYILEGLNCYSASNALACYS
4   PHEILETYRGLNARGLYSTYRASNLYSTHR
5   ARGPROVALTHRALAVALASNLYSTYRGLN
6   LYSARGLYSLEULYSVALGLNGLUTHRASN
7   GLYVALASPSERPHE

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Entity 3, DNA - Formula weight is not available

1   DCDGDGDADADADADGDTDA
2   DTDADCDTDTDTDTDCDCDG

Related Database Links:

PDB 2KAE
EMBL CAA92204 CCD72619
GB AAG21385 AAG21386
REF NP_498497 NP_510084

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