BMRB Entry 17064

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17064

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Title:
13C, and 15N Chemical Shift Assignments for Anabaena sensory rhodopsin 1-229
Deposition date:
2010-07-16
Original release date:
2011-01-19
Authors:
Shi, Lichi; Kawamura, Izuru; Jung, Kwang-Hwang; Brown, Leonid; Ladizhansky, Vladimir
Citation:

Citation: Shi, Lichi; Kawamura, Izuru; Jung, Kwang-Hwan; Brown, Leonid; Ladizhansky, Vladimir. "Conformation of a Seven-Helical Transmembrane Photosensor in the Lipid Environment."  Angew. Chem. Int. Ed. Engl. ., .-. (2010).
PubMed: 21191921

Assembly members:

Assembly members:
opsin, polymer, 235 residues, 27220 Da.
RET, non-polymer, 284.436 Da.

Natural source:

Natural source:   Common Name: Anabaena PCC7120   Taxonomy ID: 103690   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Nostoc not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: na

Entity Sequences (FASTA):

Entity Sequences (FASTA):
opsin: MNLESLLHWIYVAGMTIGAL HFWSLSRNPRGVPQYEYLVA MFIPIWSGLAYMAMAIDQGK VEAAGQIAHYARYIDWMVTT PLLLLSLSWTAMQFIKKDWT LIGFLMSTQIVVITSGLIAD LSERDWVRYLWYICGVCAFL IILWGIWNPLRAKTRTQSSE LANLYDKLVTYFTVLWIGYP IVWIIGPSGFGWINQTIDTF LFCLLPFFSKVGFSFLDLHG LRNLNDSRQHHHHHH

Data sets:
Data typeCount
13C chemical shifts668
15N chemical shifts177

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1opsin1
2retinal2

Entities:

Entity 1, opsin 235 residues - 27220 Da.

1   METASNLEUGLUSERLEULEUHISTRPILE
2   TYRVALALAGLYMETTHRILEGLYALALEU
3   HISPHETRPSERLEUSERARGASNPROARG
4   GLYVALPROGLNTYRGLUTYRLEUVALALA
5   METPHEILEPROILETRPSERGLYLEUALA
6   TYRMETALAMETALAILEASPGLNGLYLYS
7   VALGLUALAALAGLYGLNILEALAHISTYR
8   ALAARGTYRILEASPTRPMETVALTHRTHR
9   PROLEULEULEULEUSERLEUSERTRPTHR
10   ALAMETGLNPHEILELYSLYSASPTRPTHR
11   LEUILEGLYPHELEUMETSERTHRGLNILE
12   VALVALILETHRSERGLYLEUILEALAASP
13   LEUSERGLUARGASPTRPVALARGTYRLEU
14   TRPTYRILECYSGLYVALCYSALAPHELEU
15   ILEILELEUTRPGLYILETRPASNPROLEU
16   ARGALALYSTHRARGTHRGLNSERSERGLU
17   LEUALAASNLEUTYRASPLYSLEUVALTHR
18   TYRPHETHRVALLEUTRPILEGLYTYRPRO
19   ILEVALTRPILEILEGLYPROSERGLYPHE
20   GLYTRPILEASNGLNTHRILEASPTHRPHE
21   LEUPHECYSLEULEUPROPHEPHESERLYS
22   VALGLYPHESERPHELEUASPLEUHISGLY
23   LEUARGASNLEUASNASPSERARGGLNHIS
24   HISHISHISHISHIS

Entity 2, retinal - C20 H28 O - 284.436 Da.

1   RET

Related Database Links:

BMRB 18595 25290
PDB 1XIO 2M3G 4TL3
DBJ BAB74864
REF WP_010997316