BMRB Entry 17280

Assembly:

Entities:

Entity 1, BvR159 142 residues - 15895.376 Da.

Samples:

NC: BvR159, [U-98% 13C; U-98% 15N], 0.78 mM; sodium chloride 100 mM; DTT 5 mM; sodium azide 0.02%; TRIS 10 mM; D2O 5%; H2O 95%

NC5: BvR159, [5% U-98% 13C; U-98% 15N], 0.66 mM; sodium chloride 100 mM; DTT 5 mM; sodium azide 0.02%; TRIS 10 mM; D2O 5%; H2O 95%

NC5-RDC: BvR159, [5% U-98% 13C; U-98% 15N], 0.61 mM; sodium chloride 100 mM; DTT 5 mM; sodium azide 0.02%; TRIS 10 mM; D2O 5%; H2O 95%; PAGE 7%

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - geometry optimization, refinemen, structure solution

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - geometry optimization, refinement, structure solution

AutoStruct v2.1, Huang, Tejero, Powers and Montelione - data analysis, refinement

AutoAssign v2.1, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment, data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA, Keller et al. - chemical shift assignment, data analysis, peak picking

TOPSPIN, Bruker Biospin - collection

VNMRJ, Varian - collection

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

TALOS+, Shen, Cornilescu, Delaglio and Bax - geometry optimization

PALES, PALES (Zweckstetter, Bax) - geometry optimization

PROSA, Guntert - processing

NMR spectrometers:

• Bruker Avance 600 MHz
• Varian INOVA 750 MHz