BMRB Entry 17354

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PDB ID: 2lmv
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17354
MolProbity Validation Chart

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Title:
high calcium androcam
Deposition date:
2010-12-08
Original release date:
2012-07-25
Authors:
Joshi, Mehul; Moran, Sean; MacKenzie, Kevin
Citation:

Citation: Joshi, Mehul; Moran, Sean; MacKenzie, Kevin. "H, 15N and 13C chemical shifts of the D. melanogaster myosin VI light chain androcam in high calcium."  Biomol. NMR Assignments 7, 171-174 (2013).
PubMed: 22706934

Assembly members:

Assembly members:
androcam, polymer, 148 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
androcam: MSELTEEQIAEFKDAFVQFD KEGTGKIATRELGTLMRTLG QNPTEAELQDLIAEAENNNN GQLNFTEFCGIMAKQMRETD TEEEMREAFKIFDRDGDGFI SPAELRFVMINLGEKVTDEE IDEMIREADFDGDGMINYEE FVWMISQK

Data sets:
Data typeCount
13C chemical shifts587
15N chemical shifts162
1H chemical shifts1038

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1androcam1
2calcium ions2

Entities:

Entity 1, androcam 148 residues - Formula weight is not available

1   METSERGLULEUTHRGLUGLUGLNILEALA
2   GLUPHELYSASPALAPHEVALGLNPHEASP
3   LYSGLUGLYTHRGLYLYSILEALATHRARG
4   GLULEUGLYTHRLEUMETARGTHRLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   LEUILEALAGLUALAGLUASNASNASNASN
7   GLYGLNLEUASNPHETHRGLUPHECYSGLY
8   ILEMETALALYSGLNMETARGGLUTHRASP
9   THRGLUGLUGLUMETARGGLUALAPHELYS
10   ILEPHEASPARGASPGLYASPGLYPHEILE
11   SERPROALAGLULEUARGPHEVALMETILE
12   ASNLEUGLYGLULYSVALTHRASPGLUGLU
13   ILEASPGLUMETILEARGGLUALAASPPHE
14   ASPGLYASPGLYMETILEASNTYRGLUGLU
15   PHEVALTRPMETILESERGLNLYS

Entity 2, calcium ions - Ca - 40.078 Da.

1   CA

Related Database Links:

BMRB 17353
PDB 2LMT 2LMU 2LMV
EMBL CAA53630
GB AAF56511 AAL48405 ACL87451 ACL91891 ACZ95031
PRF 2021248D
REF NP_001163737 NP_476988 XP_001981747 XP_002041300 XP_002098987
SP P49258
AlphaFold P49258

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