BMRB Entry 17431

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PDB ID: 2l8y
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17431
MolProbity Validation Chart

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Title:
Solution structure of the E. coli outer membrane protein RcsF (periplasmatic domain)
Deposition date:
2011-01-27
Original release date:
2011-04-08
Authors:
Rogov, Vladimir; Doetsch, Volker; Rogova, Natalia; Bernhard, Frank; Loehr, Frank
Citation:

Citation: Rogov, Vladimir; Rogova, Natalia Yu; Bernhard, Frank; Lohr, Frank; Dotsch, Volker. "A Disulfide Bridge Network within the Soluble Periplasmic Domain Determines Structure and Function of the Outer Membrane Protein RCSF."  J. Biol. Chem. 286, 18775-18783 (2011).
PubMed: 21471196

Assembly members:

Assembly members:
RcsF, polymer, 105 residues, 11121.772 Da.

Natural source:

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: p60m

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RcsF: MAPQPKAEPAKPKAPRATPV RIYTNAEELVGKPFRDLGEV SGDSCQASNQDSPPSIPTAR KRMQINASKMKANAVLLHSC EVTSGTPGCYRQAVCIGSAL NITAK

Data sets:
Data typeCount
13C chemical shifts403
15N chemical shifts86
1H chemical shifts612

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1RcsF1

Entities:

Entity 1, RcsF 105 residues - 11121.772 Da.

Residues 1 represent a non-native cloning artefact

1   METALAPROGLNPROLYSALAGLUPROALA
2   LYSPROLYSALAPROARGALATHRPROVAL
3   ARGILETYRTHRASNALAGLUGLULEUVAL
4   GLYLYSPROPHEARGASPLEUGLYGLUVAL
5   SERGLYASPSERCYSGLNALASERASNGLN
6   ASPSERPROPROSERILEPROTHRALAARG
7   LYSARGMETGLNILEASNALASERLYSMET
8   LYSALAASNALAVALLEULEUHISSERCYS
9   GLUVALTHRSERGLYTHRPROGLYCYSTYR
10   ARGGLNALAVALCYSILEGLYSERALALEU
11   ASNILETHRALALYS

Related Database Links:

PDB 2L8Y 2Y1B
DBJ BAA03656 BAA77873 BAB33621 BAG75722 BAI23558
EMBL CAP74766 CAQ30711 CAQ87800 CAQ97085 CAR01572
GB AAA24508 AAB08624 AAC73307 AAG54498 AAN41849
REF NP_308225 NP_414738 NP_706142 WP_001202318 WP_001202319
SP P69411 P69412
AlphaFold P69411 P69412

Download HSQC peak lists in one of the following formats:
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