BMRB Entry 17519

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PDB ID: 2lab
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17519
MolProbity Validation Chart

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Title:
Solution Strucuture of CBM25-2 of beta/alpha-amylase from Paenibacillus polymyxa
Deposition date:
2011-03-09
Original release date:
2012-08-07
Authors:
Takahashi, Ryosuke; Yoshida, Takuya; Ohkubo, Tadayasu; Sumitani, Jun-ichi; Nishimura, Shigenori
Citation:

Citation: Takahashi, Ryosuke; Horibe, Ippei; Fukada, Harumi; Yoshida, Takuya; Ohkubo, Tadayasu; Inui, Takashi; Nishimura, Shigenori; Sumitani, Jun-ichi. "A functional and structural analysis of tundem family 25 carbohydrate-binding modules from Paenibacillus polymyxa beta/alpha-amylase"  Protein Sci. ., .-..

Assembly members:

Assembly members:
carbohydrate_binding_module, polymer, 104 residues, 10844.803 Da.

Natural source:

Natural source:   Common Name: Bacillus polymyxa   Taxonomy ID: 1406   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus polymyxa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
carbohydrate_binding_module: GGTTNKVTVYYKKGFNSPYI HYRPAGGSWTAAPGVKMQDA EISGYAKITVDIGSASQLEA AFNDGNNNWDSNNTKNYLFS TGTSTYTPGSNGAAGTIRTG APSG

Data sets:
Data typeCount
13C chemical shifts398
15N chemical shifts112
1H chemical shifts595

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Starch Binding Domain 2 of beta/alpha-amylase1

Entities:

Entity 1, Starch Binding Domain 2 of beta/alpha-amylase 104 residues - 10844.803 Da.

1   GLYGLYTHRTHRASNLYSVALTHRVALTYR
2   TYRLYSLYSGLYPHEASNSERPROTYRILE
3   HISTYRARGPROALAGLYGLYSERTRPTHR
4   ALAALAPROGLYVALLYSMETGLNASPALA
5   GLUILESERGLYTYRALALYSILETHRVAL
6   ASPILEGLYSERALASERGLNLEUGLUALA
7   ALAPHEASNASPGLYASNASNASNTRPASP
8   SERASNASNTHRLYSASNTYRLEUPHESER
9   THRGLYTHRSERTHRTYRTHRPROGLYSER
10   ASNGLYALAALAGLYTHRILEARGTHRGLY
11   ALAPROSERGLY

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