BMRB Entry 17529
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PDB ID: 2laj
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17529
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Aragon, Eric; Goerner, Nina; Zaromytidou, Alexia-Ileana; Xi, Qiaoran; Escobedo, Albert; Massague, Joan; Macias, Maria. "A Smad action turnover switch operated by WW domain readers of a phosphoserine code." Genes Dev. 25, 1275-1288 (2011).
PubMed: 21685363
Assembly members:
NEDD4LWW3, polymer, 44 residues, 4822.491 Da.
SMAD3, polymer, 10 residues, 1112.977 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: petM11
Entity Sequences (FASTA):
NEDD4LWW3: GAMEQSFLPPGWEMRIAPNG
RPFFYDHNTKTTTWEDPRLK
FPVH
SMAD3: AGXPNLXPNP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 61 |
| 15N chemical shifts | 22 |
| 1H chemical shifts | 276 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NEDD4LWW3 | 1 |
| 2 | SMAD3 | 2 |
Entities:
Entity 1, NEDD4LWW3 44 residues - 4822.491 Da.
| 1 | GLY | ALA | MET | GLU | GLN | SER | PHE | LEU | PRO | PRO | ||||
| 2 | GLY | TRP | GLU | MET | ARG | ILE | ALA | PRO | ASN | GLY | ||||
| 3 | ARG | PRO | PHE | PHE | TYR | ASP | HIS | ASN | THR | LYS | ||||
| 4 | THR | THR | THR | TRP | GLU | ASP | PRO | ARG | LEU | LYS | ||||
| 5 | PHE | PRO | VAL | HIS |
Entity 2, SMAD3 10 residues - 1112.977 Da.
| 1 | ALA | GLY | SEP | PRO | ASN | LEU | SEP | PRO | ASN | PRO |
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