BMRB Entry 17530

Name: Solution NMR structure of the AHSA1-like protein RHE_CH02687 (1-152) from Rhizobium etli, Northeast Structural Genomics Consortium Target ReR242
Published: 2011-04-11

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PDB ID: 2lak
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17530
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR structure of the AHSA1-like protein RHE_CH02687 (1-152) from Rhizobium etli, Northeast Structural Genomics Consortium Target ReR242

Deposition date:

2011-03-16

Original release date:

2011-04-11

Authors:

Yang, Yunhuang; Ramelot, Theresa; Cort, John; Wang, Dongyan; Ciccosanti, Colleen; Janjua, Haleema; Rost, Nair; Acton, Thomas; Xiao, Rong; Everett, John; Montelione, Gaetano; Kennedy, Michael

Citation:

Citation: Yang, Yunhuang; Ramelot, Theresa; Cort, John; Wang, Dongyan; Ciccosanti, Colleen; Janjua, Haleema; Rost, Nair; Acton, Thomas; Xiao, Rong; Everett, John; Montelione, Gaetano; Kennedy, Michael. "Solution NMR structure of the AHSA1-like protein RHE_CH02687 (1-152) from Rhizobium etli, Northeast Structural Genomics Consortium Target ReR242" .

Assembly members:

Assembly members:
AHSA1-like_protein_RHE_CH02687_(1-152)_from_Rhizobium_etli, polymer, 160 residues, 17575.8 Da.

Natural source:

Natural source: Common Name: Rhizobium etli Taxonomy ID: 29449 Superkingdom: Bacteria Kingdom: not available Genus/species: Rhizobium etli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 21-23C

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AHSA1-like_protein_RHE_CH02687_(1-152)_from_Rhizobium_etli: MEKAMPESFVVRREAHLAAP PAAVFALMTDPEKILRWMGT EAEVEPEPGGLYLVNVTGAR FARGSFREVVPVHRLAYSFG WDGSEVVPPGSSLVEIDLIE QGGGTLLRLTHSGLPSAEQC AGHEEGWAHYLGRLTEVAAG RDPGPDPFYGRRLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	639
15N chemical shifts	154
1H chemical shifts	1017

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AHSA1-like protein RHE_CH02687 (1-152)	1

Entities:

Entity 1, AHSA1-like protein RHE_CH02687 (1-152) 160 residues - 17575.8 Da.

1	MET	GLU	LYS	ALA	MET	PRO	GLU	SER	PHE	VAL
2	VAL	ARG	ARG	GLU	ALA	HIS	LEU	ALA	ALA	PRO
3	PRO	ALA	ALA	VAL	PHE	ALA	LEU	MET	THR	ASP
4	PRO	GLU	LYS	ILE	LEU	ARG	TRP	MET	GLY	THR
5	GLU	ALA	GLU	VAL	GLU	PRO	GLU	PRO	GLY	GLY
6	LEU	TYR	LEU	VAL	ASN	VAL	THR	GLY	ALA	ARG
7	PHE	ALA	ARG	GLY	SER	PHE	ARG	GLU	VAL	VAL
8	PRO	VAL	HIS	ARG	LEU	ALA	TYR	SER	PHE	GLY
9	TRP	ASP	GLY	SER	GLU	VAL	VAL	PRO	PRO	GLY
10	SER	SER	LEU	VAL	GLU	ILE	ASP	LEU	ILE	GLU
11	GLN	GLY	GLY	GLY	THR	LEU	LEU	ARG	LEU	THR
12	HIS	SER	GLY	LEU	PRO	SER	ALA	GLU	GLN	CYS
13	ALA	GLY	HIS	GLU	GLU	GLY	TRP	ALA	HIS	TYR
14	LEU	GLY	ARG	LEU	THR	GLU	VAL	ALA	ALA	GLY
15	ARG	ASP	PRO	GLY	PRO	ASP	PRO	PHE	TYR	GLY
16	ARG	ARG	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	NC_sample	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	NC_sample	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	NC_sample	isotropic	sample_conditions_1
2D 1H-15N HSQC	NC_sample_in_D2O	isotropic	sample_conditions_1
2D 1H-13C HSQC	NC_sample_in_D2O	isotropic	sample_conditions_1
2D 1H-13C HSQC-CT	NC5_sample	isotropic	sample_conditions_1
3D 1H-15N NOESY	NC5_sample	isotropic	sample_conditions_1
3D 1H-13C NOESY_aliph	NC_sample	isotropic	sample_conditions_1
3D HNCO	NC_sample	isotropic	sample_conditions_1
3D HNCACB	NC_sample	isotropic	sample_conditions_1
3D CBCA(CO)NH	NC_sample	isotropic	sample_conditions_1
3D HNCA	NC_sample	isotropic	sample_conditions_1
3D HN(CO)CA	NC_sample	isotropic	sample_conditions_1
3D HBHA(CO)NH	NC_sample	isotropic	sample_conditions_1
3D H(CCO)NH	NC_sample	isotropic	sample_conditions_1
3D C(CCO)NH	NC_sample	isotropic	sample_conditions_1
3D HCCH-COSY	NC_sample	isotropic	sample_conditions_1
3D HCCH-TOCSY	NC_sample	isotropic	sample_conditions_1
3D CCH-TOCSY	NC_sample_in_D2O	isotropic	sample_conditions_1
4D CC-NOESY	NC_sample_in_D2O	isotropic	sample_conditions_1
2D 1H-13C HSQC-aromatic	NC_sample	isotropic	sample_conditions_1

PDB	2LAK
GB	ABC91458 AGS22491
REF	WP_041678693

BMRB Entry 17530

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	MET	GLU	LYS	ALA	MET	PRO	GLU	SER	PHE	VAL
2	VAL	ARG	ARG	GLU	ALA	HIS	LEU	ALA	ALA	PRO
3	PRO	ALA	ALA	VAL	PHE	ALA	LEU	MET	THR	ASP
4	PRO	GLU	LYS	ILE	LEU	ARG	TRP	MET	GLY	THR
5	GLU	ALA	GLU	VAL	GLU	PRO	GLU	PRO	GLY	GLY
6	LEU	TYR	LEU	VAL	ASN	VAL	THR	GLY	ALA	ARG
7	PHE	ALA	ARG	GLY	SER	PHE	ARG	GLU	VAL	VAL
8	PRO	VAL	HIS	ARG	LEU	ALA	TYR	SER	PHE	GLY
9	TRP	ASP	GLY	SER	GLU	VAL	VAL	PRO	PRO	GLY
10	SER	SER	LEU	VAL	GLU	ILE	ASP	LEU	ILE	GLU
11	GLN	GLY	GLY	GLY	THR	LEU	LEU	ARG	LEU	THR
12	HIS	SER	GLY	LEU	PRO	SER	ALA	GLU	GLN	CYS
13	ALA	GLY	HIS	GLU	GLU	GLY	TRP	ALA	HIS	TYR
14	LEU	GLY	ARG	LEU	THR	GLU	VAL	ALA	ALA	GLY
15	ARG	ASP	PRO	GLY	PRO	ASP	PRO	PHE	TYR	GLY
16	ARG	ARG	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

1	MET	GLU	LYS	ALA	MET	PRO	GLU	SER	PHE	VAL
2	VAL	ARG	ARG	GLU	ALA	HIS	LEU	ALA	ALA	PRO
3	PRO	ALA	ALA	VAL	PHE	ALA	LEU	MET	THR	ASP
4	PRO	GLU	LYS	ILE	LEU	ARG	TRP	MET	GLY	THR
5	GLU	ALA	GLU	VAL	GLU	PRO	GLU	PRO	GLY	GLY
6	LEU	TYR	LEU	VAL	ASN	VAL	THR	GLY	ALA	ARG
7	PHE	ALA	ARG	GLY	SER	PHE	ARG	GLU	VAL	VAL
8	PRO	VAL	HIS	ARG	LEU	ALA	TYR	SER	PHE	GLY
9	TRP	ASP	GLY	SER	GLU	VAL	VAL	PRO	PRO	GLY
10	SER	SER	LEU	VAL	GLU	ILE	ASP	LEU	ILE	GLU
11	GLN	GLY	GLY	GLY	THR	LEU	LEU	ARG	LEU	THR
12	HIS	SER	GLY	LEU	PRO	SER	ALA	GLU	GLN	CYS
13	ALA	GLY	HIS	GLU	GLU	GLY	TRP	ALA	HIS	TYR
14	LEU	GLY	ARG	LEU	THR	GLU	VAL	ALA	ALA	GLY
15	ARG	ASP	PRO	GLY	PRO	ASP	PRO	PHE	TYR	GLY
16	ARG	ARG	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

1	MET	GLU	LYS	ALA	MET	PRO	GLU	SER	PHE	VAL
2	VAL	ARG	ARG	GLU	ALA	HIS	LEU	ALA	ALA	PRO
3	PRO	ALA	ALA	VAL	PHE	ALA	LEU	MET	THR	ASP
4	PRO	GLU	LYS	ILE	LEU	ARG	TRP	MET	GLY	THR
5	GLU	ALA	GLU	VAL	GLU	PRO	GLU	PRO	GLY	GLY
6	LEU	TYR	LEU	VAL	ASN	VAL	THR	GLY	ALA	ARG
7	PHE	ALA	ARG	GLY	SER	PHE	ARG	GLU	VAL	VAL
8	PRO	VAL	HIS	ARG	LEU	ALA	TYR	SER	PHE	GLY
9	TRP	ASP	GLY	SER	GLU	VAL	VAL	PRO	PRO	GLY
10	SER	SER	LEU	VAL	GLU	ILE	ASP	LEU	ILE	GLU
11	GLN	GLY	GLY	GLY	THR	LEU	LEU	ARG	LEU	THR
12	HIS	SER	GLY	LEU	PRO	SER	ALA	GLU	GLN	CYS
13	ALA	GLY	HIS	GLU	GLU	GLY	TRP	ALA	HIS	TYR
14	LEU	GLY	ARG	LEU	THR	GLU	VAL	ALA	ALA	GLY
15	ARG	ASP	PRO	GLY	PRO	ASP	PRO	PHE	TYR	GLY
16	ARG	ARG	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS