BMRB Entry 17535

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PDB ID: 2lar
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17535
MolProbity Validation Chart

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Title:
DNA / RNA Hybrid containing a central stereo specific Rp borano phosphate linkage
Deposition date:
2011-03-18
Original release date:
2011-05-12
Authors:
Johnson, Christopher; Spring, Alexander; Shaw, Barbara; Germann, Markus
Citation:

Citation: Johnson, Christopher; Spring, Alexander; Sergueev, Dimitri; Shaw, Barbara; Germann, Markus. "Structural Basis of the RNase H1 Activity on Stereo Regular Borano Phosphonate DNA/RNA Hybrids."  Biochemistry 50, 3903-3912 (2011).
PubMed: 21443203

Assembly members:

Assembly members:
DNA_(5'-D(*AP*TP*GP*GP*TP*BGR*CP*TP*C)-3')_, polymer, 9 residues, 2417.623 Da.
RNA_(5'-R(*GP*AP*GP*CP*AP*CP*CP*AP*U)-3')_, polymer, 9 residues, 2854.802 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DNA_(5'-D(*AP*TP*GP*GP*TP*BGR*CP*TP*C)-3')_: ATGGTXCTC
RNA_(5'-R(*GP*AP*GP*CP*AP*CP*CP*AP*U)-3')_: GAGCACCAU

Data sets:
Data typeCount
11B chemical shifts1
13C chemical shifts36
1H chemical shifts130
31P chemical shifts16

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA_chain_A1
2RNA_chain_B2

Entities:

Entity 1, DNA_chain_A 9 residues - 2417.623 Da.

1   DADTDGDGDTBGRDCDTDC

Entity 2, RNA_chain_B 9 residues - 2854.802 Da.

1   GAGCACCAU