BMRB Entry 17540

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PDB ID: 2lay
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17540
MolProbity Validation Chart

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Title:
Structure of the first WW domain of human YAP in complex with a mono-phosphorylated human Smad1 derived peptide
Deposition date:
2011-03-22
Original release date:
2011-06-23
Authors:
Macias, Maria; Aragon, Eric; Goerner, Nina; Zaromytidou, Alexia-Ileana; Xi, Qiaoran; Escobedo, Albert; Massague, Joan
Citation:

Citation: Aragon, Eric; Goerner, Nina; Zaromytidou, Alexia-Ileana; Xi, Qiaoran; Escobedo, Albert; Massague, Joan; Macias, Maria. "A Smad action turnover switch operated by WW domain readers of a phosphoserine code."  Genes Dev. 25, 1275-1288 (2011).
PubMed: 21685363

Assembly members:

Assembly members:
human Yap, polymer, 36 residues, 4151.635 Da.
human Smad1 derived peptide, polymer, 9 residues, 1056.996 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
human Yap: DVPLPAGWEMAKTSSGQRYF LNHIDQTTTWQDPRKA
human Smad1 derived peptide: STYPHXPTS

Data sets:
Data typeCount
13C chemical shifts62
15N chemical shifts28
1H chemical shifts231

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1human Yap1
2human Smad1 derived peptide2

Entities:

Entity 1, human Yap 36 residues - 4151.635 Da.

1   ASPVALPROLEUPROALAGLYTRPGLUMET
2   ALALYSTHRSERSERGLYGLNARGTYRPHE
3   LEUASNHISILEASPGLNTHRTHRTHRTRP
4   GLNASPPROARGLYSALA

Entity 2, human Smad1 derived peptide 9 residues - 1056.996 Da.

1   SERTHRTYRPROHISSEPPROTHRSER

Related Database Links:

BMRB 17539 18499
PDB 1JMQ 1K5R 1K9Q 1K9R 2LAX 2LAY 2LTW 4REX
DBJ BAE34009 BAJ41471
EMBL CAA56672
GB EAW67012 EAW67014 EPY79954
REF NP_001269026 NP_001269027 NP_001269028 NP_006097 XP_002799821
AlphaFold Q9UFT8

Download HSQC peak lists in one of the following formats:
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