BMRB Entry 17544
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PDB ID: 2lb2
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17544
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Aragon, Eric; Goerner, Nina; Zaromytidou, Alexia-Ileana; Xi, Qiaoran; Escobedo, Albert; Massague, Joan; Macias, Maria. "A Smad action turnover switch operated by WW domain readers of a phosphoserine code." Genes Dev. 25, 1275-1288 (2011).
PubMed: 21685363
Assembly members:
second domain of human Nedd4L, polymer, 35 residues, 4184.715 Da.
doubly phosphorylated human Smad3, polymer, 12 residues, 1341.284 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM11
Entity Sequences (FASTA):
second domain of human Nedd4L: GLPSGWEERKDAKGRTYYVN
HNNRTTTWTRPIMQL
doubly phosphorylated human Smad3: EXPPPGYLSEDG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 98 |
| 15N chemical shifts | 33 |
| 1H chemical shifts | 313 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Nedd4L | 1 |
| 2 | Smad3 | 2 |
Entities:
Entity 1, Nedd4L 35 residues - 4184.715 Da.
| 1 | GLY | LEU | PRO | SER | GLY | TRP | GLU | GLU | ARG | LYS | ||||
| 2 | ASP | ALA | LYS | GLY | ARG | THR | TYR | TYR | VAL | ASN | ||||
| 3 | HIS | ASN | ASN | ARG | THR | THR | THR | TRP | THR | ARG | ||||
| 4 | PRO | ILE | MET | GLN | LEU |
Entity 2, Smad3 12 residues - 1341.284 Da.
| 1 | GLU | TPO | PRO | PRO | PRO | GLY | TYR | LEU | SER | GLU | ||||
| 2 | ASP | GLY |
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