BMRB Entry 17545

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PDB ID: 2lb3
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17545
MolProbity Validation Chart

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Title:
Structure of the first domain of human PIN1 in complex with a human Smad3 derived peptide.
Deposition date:
2011-03-22
Original release date:
2011-06-23
Authors:
Macias, Maria; Aragon, Eric; Goerner, Nina; Zaromytidou, Alexia-Ileana; Xi, Qiaoran; Escobedo, Albert; Massague, Joan
Citation:

Citation: Aragon, Eric; Goerner, Nina; Zaromytidou, Alexia-Ileana; Xi, Qiaoran; Escobedo, Albert; Massague, Joan; Macias, Maria. "A Smad action turnover switch operated by WW domain readers of a phosphoserine code."  Genes Dev. 25, 1275-1288 (2011).
PubMed: 21685363

Assembly members:

Assembly members:
first domain of human PIN1, polymer, 36 residues, 4231.730 Da.
human Smad3 derived peptide, polymer, 8 residues, 886.891 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
first domain of human PIN1: KLPPGWEKRMSRSSGRVYYF NHITNASQWERPSGNS
human Smad3 derived peptide: IPEXPPPG

Data sets:
Data typeCount
13C chemical shifts65
15N chemical shifts42
1H chemical shifts269

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PIN11
2Smad32

Entities:

Entity 1, PIN1 36 residues - 4231.730 Da.

1   LYSLEUPROPROGLYTRPGLULYSARGMET
2   SERARGSERSERGLYARGVALTYRTYRPHE
3   ASNHISILETHRASNALASERGLNTRPGLU
4   ARGPROSERGLYASNSER

Entity 2, Smad3 8 residues - 886.891 Da.

1   ILEPROGLUTPOPROPROPROGLY

Related Database Links:

BMRB 16070 16088 19258 19259 25569
PDB 1F8A 1I6C 1I8G 1I8H 1NMV 1PIN 2ITK 2KCF 2LB3 2M8I 2M8J 2Q5A 2XP3 2XP4 2XP5 2XP6 2XP7 2XP8 2XP9 2XPA 2XPB 2ZQS 2ZQT 2ZQU 2ZQV 2ZR4 2ZR5 2ZR6 3KAB 3KAD 3KAF 3KAG 3KAH 3KAI 3KCE 3NTP 3ODK 3OOB 3TC5 3TCZ 3TDB 3WH0 4GWT 4GWV 4QIB 4U84 4U85 4U86
DBJ BAA87037 BAA87038 BAB22270 BAB22743 BAC35631
EMBL CAG28582
GB AAC50492 AAH02899 AAH38254 AAI12584 AAV38138
PRF 2209428A
REF NP_001029804 NP_001231300 NP_001270625 NP_006212 NP_075860
SP Q13526 Q4R383 Q5BIN5 Q9QUR7
TPG DAA28013
AlphaFold Q13526 Q4R383 Q5BIN5 Q9QUR7 Q15796

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