BMRB Entry 17582

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PDB ID: 2m3u
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17582
MolProbity Validation Chart

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Title:
1H, 13C, and 15N assignments of cataract-related variant gamma-S-G18V crystallin
Deposition date:
2011-04-08
Original release date:
2011-05-12
Authors:
Brubaker, William; Martin, Rachel
Citation:

Citation: Brubaker, William; Freites, J. Alfredo; Golchert, Kory; Shapiro, Rebecca; Morikis, Vasilios; Tobias, Douglas; Martin, Rachel. "Separating instability from aggregation propensity in S-crystallin variants."  Biophys. J. 100, 498-506 (2011).
PubMed: 21244846

Assembly members:

Assembly members:
gamma-S-G18V, polymer, 178 residues, 20974.6 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
gamma-S-G18V: GSKTGTKITFYEDKNFQVRR YDCDCDCADFHTYLSRCNSI KVEGGTWAVYERPNFAGYMY ILPQGEYPEYQRWMGLNDRL SSCRAVHLPSGGQYKIQIFE KGDFSGQMYETTEDCPSIME QFHMREIHSCKVLEGVWIFY ELPNYRGRQYLLDKKEYRKP IDWGAASPAVQSFRRIVE

Data sets:
Data typeCount
13C chemical shifts718
15N chemical shifts191
1H chemical shifts1092

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1gamma-S-G18V1

Entities:

Entity 1, gamma-S-G18V 178 residues - 20974.6 Da.

Full length human gamma-S crystallin containing the G18V substitution, and an M1G substitution.

1   GLYSERLYSTHRGLYTHRLYSILETHRPHE
2   TYRGLUASPLYSASNPHEGLNVALARGARG
3   TYRASPCYSASPCYSASPCYSALAASPPHE
4   HISTHRTYRLEUSERARGCYSASNSERILE
5   LYSVALGLUGLYGLYTHRTRPALAVALTYR
6   GLUARGPROASNPHEALAGLYTYRMETTYR
7   ILELEUPROGLNGLYGLUTYRPROGLUTYR
8   GLNARGTRPMETGLYLEUASNASPARGLEU
9   SERSERCYSARGALAVALHISLEUPROSER
10   GLYGLYGLNTYRLYSILEGLNILEPHEGLU
11   LYSGLYASPPHESERGLYGLNMETTYRGLU
12   THRTHRGLUASPCYSPROSERILEMETGLU
13   GLNPHEHISMETARGGLUILEHISSERCYS
14   LYSVALLEUGLUGLYVALTRPILEPHETYR
15   GLULEUPROASNTYRARGGLYARGGLNTYR
16   LEULEUASPLYSLYSGLUTYRARGLYSPRO
17   ILEASPTRPGLYALAALASERPROALAVAL
18   GLNSERPHEARGARGILEVALGLU

Related Database Links:

BMRB 17576
PDB 2M3T 2M3U
DBJ BAG36855
GB AAD45901 AAF72490 AAH69478 AAH70241 ADQ32938
REF NP_060011 XP_001092090 XP_002758251 XP_003256655 XP_003894751
SP P22914
AlphaFold P22914

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