BMRB Entry 17595

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PDB ID: 2lcq
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17595
MolProbity Validation Chart

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Title:
NMR resonance assignments for an archaeal homolog of the endonuclease Nob1 involved in ribosome biogenesis
Deposition date:
2011-04-19
Original release date:
2011-12-14
Authors:
Veith, Thomas; Wurm, Jan; Duchardt-Ferner, Elke; Weis, Benjamin; Hennig, Raoul; Martin, Roman; Safferthal, Charlotta; Bohnsack, Markus; Schleiff, Enrico; Woehnert, Jens
Citation:

Citation: Veith, Thomas; Martin, Roman; Wurm, Jan; Weis, Benjamin; Duchardt-Ferner, Elke; Safferthal, Charlotta; Hennig, Raoul; Mirus, Oliver; Bohnsack, Markus; Wohnert, Jens; Schleiff, Enrico. "Structural and functional analysis of the archaeal endonuclease Nob1."  Nucleic Acids Res. 40, 3259-3274 (2012).
PubMed: 22156373

Assembly members:

Assembly members:
PhNob1, polymer, 161 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Euryarchaeotes   Taxonomy ID: 53953   Superkingdom: Archaea   Kingdom: not available   Genus/species: Pyrococcus horikoshii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PhNob1: MLRNLKKTLVLDSSVFIQGI DIEGYTTPSVVEEIKDRESK IFLESLISAGKVKIAEPSKE SIDRIIQVAKETGEVNELSK ADIEVLALAYELKGEIFSDD YNVQNIASLLGLRFRTLKRG IKKVIKWRYVCIGCGRKFST LPPGGVCPDCGSKVKLIPRK R

Data sets:
Data typeCount
13C chemical shifts704
15N chemical shifts160
1H chemical shifts1097

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1PhNob11
2Zn2

Entities:

Entity 1, PhNob1 161 residues - Formula weight is not available

1   METLEUARGASNLEULYSLYSTHRLEUVAL
2   LEUASPSERSERVALPHEILEGLNGLYILE
3   ASPILEGLUGLYTYRTHRTHRPROSERVAL
4   VALGLUGLUILELYSASPARGGLUSERLYS
5   ILEPHELEUGLUSERLEUILESERALAGLY
6   LYSVALLYSILEALAGLUPROSERLYSGLU
7   SERILEASPARGILEILEGLNVALALALYS
8   GLUTHRGLYGLUVALASNGLULEUSERLYS
9   ALAASPILEGLUVALLEUALALEUALATYR
10   GLULEULYSGLYGLUILEPHESERASPASP
11   TYRASNVALGLNASNILEALASERLEULEU
12   GLYLEUARGPHEARGTHRLEULYSARGGLY
13   ILELYSLYSVALILELYSTRPARGTYRVAL
14   CYSILEGLYCYSGLYARGLYSPHESERTHR
15   LEUPROPROGLYGLYVALCYSPROASPCYS
16   GLYSERLYSVALLYSLEUILEPROARGLYS
17   ARG

Entity 2, Zn - Zn - 65.409 Da.

1   ZN

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