BMRB Entry 17629

Name: Yeast Nbp2p SH3 domain in complex with a peptide from Ste20p
Published: 2011-06-03

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PDB ID: 2lcs
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17629
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Yeast Nbp2p SH3 domain in complex with a peptide from Ste20p

Deposition date:

2011-05-07

Original release date:

2011-06-03

Authors:

Gorelik, Maryna; Davidson, Alan

Citation:

Citation: Gorelik, Maryna; Davidson, Alan. "Not known" .

Assembly members:

Assembly members:
Nbp2_SH3, polymer, 73 residues, 7562.403 Da.
Ste20_peptide, polymer, 16 residues, 1639.933 Da.

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Nbp2_SH3: MAIVNQRAVALYDFEPENDN ELRLAEGDIVFISYKHGQGW LVAENESGSKTGLVPEEFVS YIQPELEHHHHHH
Ste20_peptide: GKFIPSRPAPKPPSSA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift__list_1

Data type	Count
13C chemical shifts	280
15N chemical shifts	71
1H chemical shifts	564

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Nbp2_SH3	1
2	Ste20_peptide	2

Entities:

Entity 1, Nbp2_SH3 73 residues - 7562.403 Da.

Residues 1-2 and 66-67 represent non-native residues obtained from cloning. Residues 68-73 represent His tag.

1

MET

ALA

ILE

VAL

ASN

GLN

ARG

ALA

VAL

ALA

2

LEU

TYR

ASP

PHE

GLU

PRO

GLU

ASN

ASP

ASN

3

GLU

LEU

ARG

LEU

ALA

GLU

GLY

ASP

ILE

VAL

4

PHE

ILE

SER

TYR

LYS

HIS

GLY

GLN

GLY

TRP

5

LEU

VAL

ALA

GLU

ASN

GLU

SER

GLY

SER

LYS

6

THR

GLY

LEU

VAL

PRO

GLU

PHE

VAL

SER

7

TYR

ILE

GLN

PRO

GLU

LEU

GLU

HIS

8

HIS

Entity 2, Ste20_peptide 16 residues - 1639.933 Da.

1

GLY

LYS

PHE

ILE

PRO

SER

ARG

PRO

ALA

PRO

2

LYS

PRO

SER

ALA

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1
N15 C13 filtered 2D 1H-1H TOCSY	sample_3	isotropic	sample_conditions_1
15N C13 filtered 2D 1H-1H NOESY	sample_3	isotropic	sample_conditions_1
13C half-filtered 3D 1H-13C NOESY	sample_4	isotropic	sample_conditions_1
13C filtered 2D 1H-1H COSY	sample_4	isotropic	sample_conditions_1

PDB	1YN8 2LCS 2KYM 2LCS 2RQW
DBJ	BAA07790 GAA22392 GAA23703
EMBL	CAA90382 CAY78665
GB	AAS56004 AHY75148 AJP37876 AJU57998 AJU58693 AAA35038 AAA35111 AAB69747 AHY77690 EDN62227
REF	NP_010446 NP_011856
SP	Q12163 Q03497
TPG	DAA12002 DAA06681
BMRB	16970
AlphaFold	Q12163 Q03497

BMRB Entry 17629

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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