BMRB Entry 17645

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PDB ID: 2ld3
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17645
MolProbity Validation Chart

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Title:
Solution structure of myosin VI lever arm extension
Deposition date:
2011-05-13
Original release date:
2012-08-30
Authors:
Feng, Wei; Yu, Cong; Zhang, Mingjie
Citation:

Citation: Yu, Cong; Lou, Jizhong; Wu, Jingjing; Pan, Lifeng; Feng, Wei; Zhang, Mingjie. "Membrane-induced lever arm expansion allows myosin VI to walk with large and variable step sizes."  J. Biol. Chem. 287, 35021-35035 (2012).
PubMed: 22936804

Assembly members:

Assembly members:
myosin_VI_LAE, polymer, 88 residues, 9961.641 Da.

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET32a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
myosin_VI_LAE: QGPGSLVKVGTLKKRLDKFN EVVSALKDGKPEVNRQIKNL EISIDALMAKIKSTMMTREQ IQKEYDALVKSSEDLLSALQ KKKQQEEE

Data sets:
Data typeCount
13C chemical shifts305
15N chemical shifts83
1H chemical shifts637

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1myosin VI lever arm extension1

Entities:

Entity 1, myosin VI lever arm extension 88 residues - 9961.641 Da.

Residues "QGPGS" are from the non-native tag

1   GLNGLYPROGLYSERLEUVALLYSVALGLY
2   THRLEULYSLYSARGLEUASPLYSPHEASN
3   GLUVALVALSERALALEULYSASPGLYLYS
4   PROGLUVALASNARGGLNILELYSASNLEU
5   GLUILESERILEASPALALEUMETALALYS
6   ILELYSSERTHRMETMETTHRARGGLUGLN
7   ILEGLNLYSGLUTYRASPALALEUVALLYS
8   SERSERGLUASPLEULEUSERALALEUGLN
9   LYSLYSLYSGLNGLNGLUGLUGLU

Related Database Links:

PDB 2LD3
DBJ BAD32219
GB AAB00194 AAI44918 AAI50674 EDL26433 EDL26434
PRF 2204386A
REF NP_001034635 XP_001061392 XP_003508949 XP_004696356 XP_004696357
SP Q64331
AlphaFold Q64331

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks