BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17667

Title: 1H, 13C & 13N chemical shift assignments for Toxoplasma gondii Microneme protein 4 (MIC4) residues 410-491 (apple domain 5)

Deposition date: 2011-05-25 Original release date: 2011-06-24

Authors: Matthews, Steve; Pete, Simpson; Jan, Marchant

Citation: Cowper, Ben. "TgMIC4-apple5 assignments"  .

Assembly members:
TgMIC4-apple5, polymer, 82 residues, Formula weight is not available

Natural source:   Common Name: Toxoplasma gondii   Taxonomy ID: 5811   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Toxoplasma gondii

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-32 Xa/LIC

Entity Sequences (FASTA):
TgMIC4-apple5: SPDFHDEVECVHTGNIGSKA QTIGEVKRASSLSECRARCQ AEKECSHYTYNVKSGLCYPK RGKPQFYKYLGDMTGSRTCD TS

Data sets:
Data typeCount
13C chemical shifts315
15N chemical shifts73
1H chemical shifts452

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TgMIC4-apple51

Entities:

Entity 1, TgMIC4-apple5 82 residues - Formula weight is not available

1   SERPROASPPHEHISASPGLUVALGLUCYS
2   VALHISTHRGLYASNILEGLYSERLYSALA
3   GLNTHRILEGLYGLUVALLYSARGALASER
4   SERLEUSERGLUCYSARGALAARGCYSGLN
5   ALAGLULYSGLUCYSSERHISTYRTHRTYR
6   ASNVALLYSSERGLYLEUCYSTYRPROLYS
7   ARGGLYLYSPROGLNPHETYRLYSTYRLEU
8   GLYASPMETTHRGLYSERARGTHRCYSASP
9   THRSER

Samples:

sample_1: TgMIC4-apple5, [U-98% 13C; U-98% 15N], 100 – 200 mM; potassium phosphate 50 mM; sodium chloride 100 mM; H20 90%; D20 10%

sample_2: TgMIC4-apple5, [U-98% 13C; U-98% 15N], 100-200 mM; potassium phosphate 50 mM; sodium chloride 100 mM; H20 90%; D20 10%

sample_conditions_1: ionic strength: 0.7 M; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_2isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts