BMRB Entry 17723

Assembly:

Entities:

Entity 1, ChR145 146 residues - 16816.273 Da.

LEHHHHHH are part of the C-terminal and not included in the coordinate.

Samples:

NC1: ChR145, [U-100% 13C; U-100% 15N], 0.85 mM; sodium azide 0.2%; DTT 5 mM; CaCl2 5 mM; sodium chloride 200 mM; MES 20 mM; H2O 95%; D2O 5%

Phage: ChR145, [U-100% 13C; U-100% 15N], 0.57 mM; sodium azide 0.2%; DTT 5 mM; CaCl2 5 mM; sodium chloride 200 mM; MES 20 mM; Pf1 phage 12.5 mg; H2O 95%; D2O 5%

Peg: ChR145, [U-100% 13C; U-100% 15N], 0.57 mM; sodium azide 0.2%; DTT 5 mM; CaCl2 5 mM; sodium chloride 200 mM; MES 20 mM; C12E5/Hexanol 4%; H2O 95%; D2O 5%

Neutral_Gel: ChR145, [U-100% 13C; U-100% 15N], 0.85 mM; sodium azide 0.2%; DTT 5 mM; CaCl2 5 mM; sodium chloride 200 mM; MES 20 mM; Polyacrylamide 5%; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 200 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

SPARKY, Goddard - chemical shift assignment, peak picking

X-PLOR NIH v2.18, Schwieters, Kuszewski, Tjandra and Clore - refinement

PSVS, Bhattacharya and Montelione - Validation

NMR spectrometers:

• Varian INOVA 600 MHz