BMRB Entry 17732

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PDB ID: 2lex
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17732
MolProbity Validation Chart

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Title:
Complex of the C-terminal WRKY domain of AtWRKY4 and a W-box DNA
Deposition date:
2011-06-24
Original release date:
2012-01-18
Authors:
Kigawa, Takanori; Watanabe, Satoru; Inoue, Makoto; Yokoyama, Shigeyuki
Citation:

Citation: Yamasaki, Kazuhiko; Kigawa, Takanori; Watanabe, Satoru; Inoue, Makoto; Yamasaki, Tomoko; Seki, Motoaki; Shinozaki, Kazuo; Yokoyama, Shigeyuki. "Structural Basis for Sequence-specific DNA Recognition by an Arabidopsis WRKY Transcription Factor."  J. Biol. Chem. 287, 7683-7691 (2012).
PubMed: 22219184

Assembly members:

Assembly members:
WRKY_domain, polymer, 78 residues, 7208.253 Da.
ZN, non-polymer, 65.409 Da.
W-box_DNA_chain_1, polymer, . residues, Formula weight is not available
W-box_DNA_chain_2, polymer, . residues, Formula weight is not available

Natural source:

Natural source:   Common Name: thale-cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: pCR2.1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
WRKY_domain: GSSGSSGVQTTSEVDLLDDG YRWRKYGQKVVKGNPYPRSY YKCTTPGCGVRKHVERAATD PKAVVTTYEGKHNHDLPA
W-box_DNA_chain_1: CGCCTTTGACCAGCGC
W-box_DNA_chain_2: GCGCTGGTCAAAGGCG

Data sets:
Data typeCount
13C chemical shifts70
15N chemical shifts80
1H chemical shifts662

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1WRKY_domain1
2ZINC_ION2
3W-box_DNA_chain_13
4W-box_DNA_chain_24

Entities:

Entity 1, WRKY_domain 78 residues - 7208.253 Da.

1   GLYSERSERGLYSERSERGLYVALGLNTHR
2   THRSERGLUVALASPLEULEUASPASPGLY
3   TYRARGTRPARGLYSTYRGLYGLNLYSVAL
4   VALLYSGLYASNPROTYRPROARGSERTYR
5   TYRLYSCYSTHRTHRPROGLYCYSGLYVAL
6   ARGLYSHISVALGLUARGALAALATHRASP
7   PROLYSALAVALVALTHRTHRTYRGLUGLY
8   LYSHISASNHISASPLEUPROALA

Entity 2, ZINC_ION - Zn - 65.409 Da.

1   ZN

Entity 3, W-box_DNA_chain_1 - Formula weight is not available

1   DCDGDCDCDTDTDTDGDADC
2   DCDADGDCDGDC

Entity 4, W-box_DNA_chain_2 - Formula weight is not available

1   DGDCDGDCDTDGDGDTDCDA
2   DADADGDGDCDG

Related Database Links:

PDB 1WJ2 2LEX

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