BMRB Entry 17754

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PDB ID: 2lfi
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17754
MolProbity Validation Chart

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Title:
Solution NMR structure of a MucBP domain (fragment 187-294) of the protein LBA1460 from Lactobacillus acidophilus, Northeast structural genomics consortium target LaR80A
Deposition date:
2011-06-30
Original release date:
2011-08-03
Authors:
Feldmann, Erik; Ramelot, Theresa; Yang, Yunhuang; Ciccosanti, Colleen; Janjua, Haleema; Nair, Rajesh; Acton, Thomas; Xiao, Rong; Everett, John; Montelione, Gaetano; Kennedy, Michael
Citation:

Citation: Feldmann, Erik; Ramelot, Theresa; Yang, Yunhuang; Wang, Huang; Ciccosanti, Colleen; Janjua, Haleema; Nair, Rajesh; Acton, Thomas; Xiao, Rong; Everett, John; Montelione, Gaetano; Kennedy, Michael. "Solution NMR structure of a MucBP domain (fragment 187-294) of the protein LBA1460 from Lactobacillus acidophilus, Northeast structural genomics consortium target LaR80A"  .

Assembly members:

Assembly members:
MucBP, polymer, 366 residues, 14000 Da.

Natural source:

Natural source:   Common Name: Lactobacillus acidophilus   Taxonomy ID: 1579   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Lactobacillus acidophilus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 21-23C

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MucBP: MIEPIKRTQVVTQTIHYRYE DGAVAHDDHVVSLIFTQSGK RDLTNGKEIWDSKWSLTQTF EALPSPVIIGYTADKPMVGP DEVTVDSKNFLDKQNREETV IYSANTITQNKKDGLEHHHH HHMIEPIKRTQVVTQTIHYR YEDGAVAHDDHVVSLIFTQS GKRDLTNGKEIWDSKWSLTQ TFEALPSPVIIGYTADKPMV GPDEVTVDSKNFLDKQNREE TVIYSANTITQNKKDGLEHH HHHHMIEPIKRTQVVTQTIH YRYEDGAVAHDDHVVSLIFT QSGKRDLTNGKEIWDSKWSL TQTFEALPSPVIIGYTADKP MVGPDEVTVDSKNFLDKQNR EETVIYSANTITQNKKDGLE HHHHHH

Data sets:
Data typeCount
13C chemical shifts513
15N chemical shifts127
1H chemical shifts821

Time Domain Data

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1MucBP1

Entities:

Entity 1, MucBP 366 residues - 14000 Da.

1   METILEGLUPROILELYSARGTHRGLNVAL
2   VALTHRGLNTHRILEHISTYRARGTYRGLU
3   ASPGLYALAVALALAHISASPASPHISVAL
4   VALSERLEUILEPHETHRGLNSERGLYLYS
5   ARGASPLEUTHRASNGLYLYSGLUILETRP
6   ASPSERLYSTRPSERLEUTHRGLNTHRPHE
7   GLUALALEUPROSERPROVALILEILEGLY
8   TYRTHRALAASPLYSPROMETVALGLYPRO
9   ASPGLUVALTHRVALASPSERLYSASNPHE
10   LEUASPLYSGLNASNARGGLUGLUTHRVAL
11   ILETYRSERALAASNTHRILETHRGLNASN
12   LYSLYSASPGLYLEUGLUHISHISHISHIS
13   HISHISMETILEGLUPROILELYSARGTHR
14   GLNVALVALTHRGLNTHRILEHISTYRARG
15   TYRGLUASPGLYALAVALALAHISASPASP
16   HISVALVALSERLEUILEPHETHRGLNSER
17   GLYLYSARGASPLEUTHRASNGLYLYSGLU
18   ILETRPASPSERLYSTRPSERLEUTHRGLN
19   THRPHEGLUALALEUPROSERPROVALILE
20   ILEGLYTYRTHRALAASPLYSPROMETVAL
21   GLYPROASPGLUVALTHRVALASPSERLYS
22   ASNPHELEUASPLYSGLNASNARGGLUGLU
23   THRVALILETYRSERALAASNTHRILETHR
24   GLNASNLYSLYSASPGLYLEUGLUHISHIS
25   HISHISHISHISMETILEGLUPROILELYS
26   ARGTHRGLNVALVALTHRGLNTHRILEHIS
27   TYRARGTYRGLUASPGLYALAVALALAHIS
28   ASPASPHISVALVALSERLEUILEPHETHR
29   GLNSERGLYLYSARGASPLEUTHRASNGLY
30   LYSGLUILETRPASPSERLYSTRPSERLEU
31   THRGLNTHRPHEGLUALALEUPROSERPRO
32   VALILEILEGLYTYRTHRALAASPLYSPRO
33   METVALGLYPROASPGLUVALTHRVALASP
34   SERLYSASNPHELEUASPLYSGLNASNARG
35   GLUGLUTHRVALILETYRSERALAASNTHR
36   ILETHRGLNASNLYSLYSASPGLYLEUGLU
37   HISHISHISHISHISHIS

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