BMRB Entry 17768

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PDB ID: 2lfp
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17768
MolProbity Validation Chart

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Title:
Structure of bacteriophage SPP1 gp17 protein
Deposition date:
2011-07-07
Original release date:
2012-05-09
Authors:
Chagot, Benjamin; Auzat, Isabelle; Gallopin, Matthieu; Petitpas, Isabelle; Gilquin, Bernard; Tavares, Paulo; Zinn-Justin, Sophie
Citation:

Citation: Chagot, Benjamin; Auzat, Isabelle; Gallopin, Matthieu; Petitpas, Isabelle; Gilquin, Bernard; Tavares, Paulo; Zinn-Justin, Sophie. "Solution structure of gp17 from the Siphoviridae bacteriophage SPP1: insights into its role in virion assembly."  Proteins 80, 319-326 (2012).
PubMed: 22072538

Assembly members:

Assembly members:
gp17, polymer, 139 residues, 15478.582 Da.

Natural source:

Natural source:   Common Name: Bacteriophage SPP1   Taxonomy ID: 10724   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lambda-like viruses Bacteriophage SPP1

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: N/A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
gp17: QGLQTWKLASRALQKATVEN LESYQPLMEMVNQVTESPGK DDPYPYVVIGDQSSTPFETK SSFGENITMDFHVWGGTTRA EAQDISSRVLEALTYKPLMF EGFTFVAKKLVLAQVITDTD GVTKHGIIKVRFTINNNTG

Data sets:
Data typeCount
13C chemical shifts282
15N chemical shifts102
1H chemical shifts708

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1gp171

Entities:

Entity 1, gp17 139 residues - 15478.582 Da.

First four residues (QGLQ) and the two last (TG) are cloning artefacts

1   GLNGLYLEUGLNTHRTRPLYSLEUALASER
2   ARGALALEUGLNLYSALATHRVALGLUASN
3   LEUGLUSERTYRGLNPROLEUMETGLUMET
4   VALASNGLNVALTHRGLUSERPROGLYLYS
5   ASPASPPROTYRPROTYRVALVALILEGLY
6   ASPGLNSERSERTHRPROPHEGLUTHRLYS
7   SERSERPHEGLYGLUASNILETHRMETASP
8   PHEHISVALTRPGLYGLYTHRTHRARGALA
9   GLUALAGLNASPILESERSERARGVALLEU
10   GLUALALEUTHRTYRLYSPROLEUMETPHE
11   GLUGLYPHETHRPHEVALALALYSLYSLEU
12   VALLEUALAGLNVALILETHRASPTHRASP
13   GLYVALTHRLYSHISGLYILEILELYSVAL
14   ARGPHETHRILEASNASNASNTHRGLY

Related Database Links:

PDB 2LFP 5A20 5A21
EMBL CAA66549
REF NP_690679

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks