BMRB Entry 17817

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17817

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Title:
Green Proteorhodopsin - Deuterated
Deposition date:
2011-07-29
Original release date:
2011-10-25
Authors:
Ward, Meaghan; Shi, Lichi; Lake, Evelyn; Krishnamurthy, Sridevi; Hutchins, Howard; Brown, Leonid; Ladizhansky, Vladimir
Citation:

Citation: Ward, Meaghan; Shi, Lichi; Lake, Evelyn; Krishnamurthy, Sridevi; Hutchins, Howard; Brown, Leonid; Ladizhansky, Vladimir. "Proton-detected solid-state NMR reveals intramembrane polar networks in a seven-helical transmembrane protein proteorhodopsin."  J. Am. Chem. Soc. 133, 17434-17443 (2011).
PubMed: 21919530

Assembly members:

Assembly members:
GPR, polymer, 255 residues, Formula weight is not available
RET, non-polymer, 284.436 Da.

Natural source:

Natural source:   Common Name: Gamma proteobacteria   Taxonomy ID: 1236   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Gamma proteobacteria

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21   Vector: NA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GPR: MKLLLILGSVIALPTFAAGG GDLDASDYTGVSFWLVTAAL LASTVFFFVERDRVSAKWKT SLTVSGLVTGIAFWHYMYMR GVWIETGDSPTVFRYIDWLL TVPLLICEFYLILAAATNVA GSLFKKLLVGSLVMLVFGYM GEAGIMAAWPAFIIGCLAWV YMIYELWAGEGKSACNTASP AVQSAYNTMMYIIIFGWAIY PVGYFTGYLMGDGGSALNLN LIYNLADFVNKILFGLIIWN VAVKESSNAHHHHHH

Data sets:
Data typeCount
13C chemical shifts195
15N chemical shifts74
1H chemical shifts74

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Green Proteorhodopsin1
2retinal2

Entities:

Entity 1, Green Proteorhodopsin 255 residues - Formula weight is not available

1   METLYSLEULEULEUILELEUGLYSERVAL
2   ILEALALEUPROTHRPHEALAALAGLYGLY
3   GLYASPLEUASPALASERASPTYRTHRGLY
4   VALSERPHETRPLEUVALTHRALAALALEU
5   LEUALASERTHRVALPHEPHEPHEVALGLU
6   ARGASPARGVALSERALALYSTRPLYSTHR
7   SERLEUTHRVALSERGLYLEUVALTHRGLY
8   ILEALAPHETRPHISTYRMETTYRMETARG
9   GLYVALTRPILEGLUTHRGLYASPSERPRO
10   THRVALPHEARGTYRILEASPTRPLEULEU
11   THRVALPROLEULEUILECYSGLUPHETYR
12   LEUILELEUALAALAALATHRASNVALALA
13   GLYSERLEUPHELYSLYSLEULEUVALGLY
14   SERLEUVALMETLEUVALPHEGLYTYRMET
15   GLYGLUALAGLYILEMETALAALATRPPRO
16   ALAPHEILEILEGLYCYSLEUALATRPVAL
17   TYRMETILETYRGLULEUTRPALAGLYGLU
18   GLYLYSSERALACYSASNTHRALASERPRO
19   ALAVALGLNSERALATYRASNTHRMETMET
20   TYRILEILEILEPHEGLYTRPALAILETYR
21   PROVALGLYTYRPHETHRGLYTYRLEUMET
22   GLYASPGLYGLYSERALALEUASNLEUASN
23   LEUILETYRASNLEUALAASPPHEVALASN
24   LYSILELEUPHEGLYLEUILEILETRPASN
25   VALALAVALLYSGLUSERSERASNALAHIS
26   HISHISHISHISHIS

Entity 2, retinal - C20 H28 O - 284.436 Da.

1   RET

Related Database Links:

BMRB 15955 17327
PDB 2L6X
GB AAG10475 AAK30175 AAK30176 AAK30181 AAK30183
SP Q9F7P4
AlphaFold Q9F7P4

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SPARKY: Backbone or all simulated peaks