BMRB Entry 17818

Name: Solution NMR structure of a FKBP-type peptidyl-prolyl cis-trans isomerase from Giardia lamblia. Seattle Structural Genomics Center for Infectious Disease (SSGCID) target GilaA.00840.a
Published: 2011-08-17

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PDB ID: 2lgo
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17818
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR structure of a FKBP-type peptidyl-prolyl cis-trans isomerase from Giardia lamblia. Seattle Structural Genomics Center for Infectious Disease (SSGCID) target GilaA.00840.a

Deposition date:

2011-07-29

Original release date:

2011-08-17

Authors:

Buchko, Garry

Citation:

Citation: Buchko, Garry; Hewitt, Stephen; Van Voorhis, Wesley; Myler, Peter. "Solution structure of a putative FKBP-type peptidyl-propyl cis-trans isomerase from Giardia lamblia." J. Biomol. NMR 57, 369-374 (2013).
PubMed: 24293257

Assembly members:

Assembly members:
FKBP, polymer, 130 residues, 14160.289 Da.

Natural source:

Natural source: Common Name: Giardia lamblia Taxonomy ID: 184922 Superkingdom: Eukaryota Kingdom: not available Genus/species: Giardia lamblia

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: AVA0421

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FKBP: MAHHHHHHMGTLEAQTQGPG SMSAQLEKKVLTPGDGVTKP QAGKKVTVHYDGRFPDGKQF DSSRSRGKPFQFTLGAGEVI KGWDQGVATMTLGEKALFTI PYQLAYGERGYPPVIPPKAT LVFEVELLAV

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	378
15N chemical shifts	116
1H chemical shifts	726

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FKBP	1

Entities:

Entity 1, FKBP 130 residues - 14160.289 Da.

The first 21 residues are a tag for protein isolation (MAHHHHHHMGTLEAQTQGPGS). The first native residue is M22.

1	MET	ALA	HIS	HIS	HIS	HIS	HIS	HIS	MET	GLY
2	THR	LEU	GLU	ALA	GLN	THR	GLN	GLY	PRO	GLY
3	SER	MET	SER	ALA	GLN	LEU	GLU	LYS	LYS	VAL
4	LEU	THR	PRO	GLY	ASP	GLY	VAL	THR	LYS	PRO
5	GLN	ALA	GLY	LYS	LYS	VAL	THR	VAL	HIS	TYR
6	ASP	GLY	ARG	PHE	PRO	ASP	GLY	LYS	GLN	PHE
7	ASP	SER	SER	ARG	SER	ARG	GLY	LYS	PRO	PHE
8	GLN	PHE	THR	LEU	GLY	ALA	GLY	GLU	VAL	ILE
9	LYS	GLY	TRP	ASP	GLN	GLY	VAL	ALA	THR	MET
10	THR	LEU	GLY	GLU	LYS	ALA	LEU	PHE	THR	ILE
11	PRO	TYR	GLN	LEU	ALA	TYR	GLY	GLU	ARG	GLY
12	TYR	PRO	PRO	VAL	ILE	PRO	PRO	LYS	ALA	THR
13	LEU	VAL	PHE	GLU	VAL	GLU	LEU	LEU	ALA	VAL

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
deuterium exchange	sample_2	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
2D HBCBCGCDCDHD	sample_1	isotropic	sample_conditions_1
2D HBCBCGCDCEHE	sample_1	isotropic	sample_conditions_1

PDB	2LGO
GB	AAM33435 EDO81467 EET00236 EFO62827 ESU38634
REF	XP_001709141

BMRB Entry 17818

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	MET	ALA	HIS	HIS	HIS	HIS	HIS	HIS	MET	GLY
2	THR	LEU	GLU	ALA	GLN	THR	GLN	GLY	PRO	GLY
3	SER	MET	SER	ALA	GLN	LEU	GLU	LYS	LYS	VAL
4	LEU	THR	PRO	GLY	ASP	GLY	VAL	THR	LYS	PRO
5	GLN	ALA	GLY	LYS	LYS	VAL	THR	VAL	HIS	TYR
6	ASP	GLY	ARG	PHE	PRO	ASP	GLY	LYS	GLN	PHE
7	ASP	SER	SER	ARG	SER	ARG	GLY	LYS	PRO	PHE
8	GLN	PHE	THR	LEU	GLY	ALA	GLY	GLU	VAL	ILE
9	LYS	GLY	TRP	ASP	GLN	GLY	VAL	ALA	THR	MET
10	THR	LEU	GLY	GLU	LYS	ALA	LEU	PHE	THR	ILE
11	PRO	TYR	GLN	LEU	ALA	TYR	GLY	GLU	ARG	GLY
12	TYR	PRO	PRO	VAL	ILE	PRO	PRO	LYS	ALA	THR
13	LEU	VAL	PHE	GLU	VAL	GLU	LEU	LEU	ALA	VAL

1	MET	ALA	HIS	HIS	HIS	HIS	HIS	HIS	MET	GLY
2	THR	LEU	GLU	ALA	GLN	THR	GLN	GLY	PRO	GLY
3	SER	MET	SER	ALA	GLN	LEU	GLU	LYS	LYS	VAL
4	LEU	THR	PRO	GLY	ASP	GLY	VAL	THR	LYS	PRO
5	GLN	ALA	GLY	LYS	LYS	VAL	THR	VAL	HIS	TYR
6	ASP	GLY	ARG	PHE	PRO	ASP	GLY	LYS	GLN	PHE
7	ASP	SER	SER	ARG	SER	ARG	GLY	LYS	PRO	PHE
8	GLN	PHE	THR	LEU	GLY	ALA	GLY	GLU	VAL	ILE
9	LYS	GLY	TRP	ASP	GLN	GLY	VAL	ALA	THR	MET
10	THR	LEU	GLY	GLU	LYS	ALA	LEU	PHE	THR	ILE
11	PRO	TYR	GLN	LEU	ALA	TYR	GLY	GLU	ARG	GLY
12	TYR	PRO	PRO	VAL	ILE	PRO	PRO	LYS	ALA	THR
13	LEU	VAL	PHE	GLU	VAL	GLU	LEU	LEU	ALA	VAL

1	MET	ALA	HIS	HIS	HIS	HIS	HIS	HIS	MET	GLY
2	THR	LEU	GLU	ALA	GLN	THR	GLN	GLY	PRO	GLY
3	SER	MET	SER	ALA	GLN	LEU	GLU	LYS	LYS	VAL
4	LEU	THR	PRO	GLY	ASP	GLY	VAL	THR	LYS	PRO
5	GLN	ALA	GLY	LYS	LYS	VAL	THR	VAL	HIS	TYR
6	ASP	GLY	ARG	PHE	PRO	ASP	GLY	LYS	GLN	PHE
7	ASP	SER	SER	ARG	SER	ARG	GLY	LYS	PRO	PHE
8	GLN	PHE	THR	LEU	GLY	ALA	GLY	GLU	VAL	ILE
9	LYS	GLY	TRP	ASP	GLN	GLY	VAL	ALA	THR	MET
10	THR	LEU	GLY	GLU	LYS	ALA	LEU	PHE	THR	ILE
11	PRO	TYR	GLN	LEU	ALA	TYR	GLY	GLU	ARG	GLY
12	TYR	PRO	PRO	VAL	ILE	PRO	PRO	LYS	ALA	THR
13	LEU	VAL	PHE	GLU	VAL	GLU	LEU	LEU	ALA	VAL