BMRB Entry 17862

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PDB ID: 2lhr
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: LACS
BMRB Entry DOI: doi:10.13018/BMR17862
MolProbity Validation Chart

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Title:
Solution structure of Staphylococcus aureus IsdH linker domain
Deposition date:
2011-08-12
Original release date:
2012-06-19
Authors:
Spirig, Thomas; Clubb, Robert; Malmirchegini, G.; Robson, Scott
Citation:

Citation: Krishna Kumar, Kaavya; Jacques, David; Pishchany, Gleb; Caradoc-Davies, Tom; Spirig, Thomas; Malmirchegini, G. Reza; Langley, David; Dickson, Claire; Mackay, Joel; Clubb, Robert; Skaar, Eric; Guss, J. Mitchell; Gell, David. "Structural basis for hemoglobin capture by Staphylococcus aureus cell-surface protein, IsdH."  J. Biol. Chem. 286, 38439-38447 (2011).
PubMed: 21917915

Assembly members:

Assembly members:
entity, polymer, 78 residues, 9280.425 Da.

Natural source:

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: -

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: SDDYVDEETYNLQKLLAPYH KAKTLERQVYELEKLQEKLP EKYKAEYKKKLDQTRVELAD QVKSAVTEFENVTPTNDQ

Data sets:
Data typeCount
13C chemical shifts357
15N chemical shifts82
1H chemical shifts568

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Staphylococcus aureus IsdH linker domain1

Entities:

Entity 1, Staphylococcus aureus IsdH linker domain 78 residues - 9280.425 Da.

Residue 1 represents a non-native residue left after cleaving of the affinity tag

1   SERASPASPTYRVALASPGLUGLUTHRTYR
2   ASNLEUGLNLYSLEULEUALAPROTYRHIS
3   LYSALALYSTHRLEUGLUARGGLNVALTYR
4   GLULEUGLULYSLEUGLNGLULYSLEUPRO
5   GLULYSTYRLYSALAGLUTYRLYSLYSLYS
6   LEUASPGLNTHRARGVALGLULEUALAASP
7   GLNVALLYSSERALAVALTHRGLUPHEGLU
8   ASNVALTHRPROTHRASNASPGLN

Related Database Links:

BMRB 17820
PDB 2LHR 4IJ2 4XS0
DBJ BAB42820 BAB57893 BAB95538 BAF67896 BAF78600
EMBL CAG43459 CAI81279 CAQ50207 CBI49597 CBX34951
GB AAW38309 ABD20516 ABD30910 ABQ49576 ABR52665
REF WP_001032759 WP_001032760 WP_001032761 WP_001032762 WP_001032763
SP Q2FG07 Q2FXJ2 Q2YTF7 Q5HF43 Q6G8J7
AlphaFold Q2FG07 Q2FXJ2 Q2YTF7 Q5HF43 Q6G8J7

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